2016 ; 26(11):
|Author||Ji-Hyun Nam, Jey-R. S. Ventura, Ick Tae Yeom, Yongwoo Lee, Deokjin Jahng|
|Affiliation||Department of Environmental Engineering and Energy, Myongji University, Yongin 17058, Republic of Korea|
|Title||Structural and Kinetic Characteristics of 1,4-Dioxane-Degrading Bacterial Consortia Containing the Phylum TM7|
J. Microbiol. Biotechnol.2016 ; 26(11):
|Abstract||1,4-Dioxane-degrading bacterial consortia were enriched from forest soil (FS) and activated
sludge (AS) using a defined medium containing 1,4-dioxane as the sole carbon source. These
two enrichments cultures appeared to have inducible tetrahydrofuran/dioxane and propane
degradation enzymes. According to qPCR results on the 16S rRNA and soluble di-iron
monooxygenase genes, the relative abundances of 1,4-dioxane-degrading bacteria to total
bacteria in FS and AS were 29.4% and 57.8%, respectively. For FS, the cell growth yields (Y),
maximum specific degradation rate (Vmax), and half-saturation concentration (Km) were
0.58 mg-protein/mg-dioxane, 0.037 mg-dioxane/mg-protein∙h, and 93.9 mg/l, respectively.
For AS, Y, Vmax, and Km were 0.34 mg-protein/mg-dioxane, 0.078 mg-dioxane/mg-protein∙h,
and 181.3 mg/l, respectively. These kinetics data of FS and AS were similar to previously
reported values. Based on bacterial community analysis on 16S rRNA gene sequences of the
two enrichment cultures, the FS consortium was identified to contain 38.3% of Mycobacterium
and 10.6% of Afipia, similar to previously reported literature. Meanwhile, 49.5% of the AS
consortium belonged to the candidate division TM7, which has never been reported to be
involved in 1,4-dioxane biodegradation. However, recent studies suggested that TM7 bacteria
were associated with degradation of non-biodegradable and hazardous materials. Therefore,
our results showed that previously unknown 1,4-dioxane-degrading bacteria might play an
important role in enriched AS. Although the metabolic capability and ecophysiological
significance of the predominant TM7 bacteria in AS enrichment culture remain unclear, our
data reveal hidden characteristics of the TM7 phylum and provide a perspective for studying
this previously uncultured phylotype.|
|Keywords||1,4-Dioxane degrading bacterial consortia, candidate division TM7, Mycobacterium, Afipia, kinetic parameters, soluble di-iron monooxygenase|
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