2016 ; 26(6):
|Author||Seoyoung Park, Joo-Hyun Kim, Younglim Son, Sung-Ho Goh, Sangtaek Oh|
|Affiliation||Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea|
|Title||Longan (Dimocarpus longan Lour.) Fruit Extract Stimulates Osteoblast Differentiation via Erk1/2-Dependent RUNX2 Activation|
J. Microbiol. Biotechnol.2016 ; 26(6):
|Abstract||Longan (Dimocarpus longan Lour.) has been used as a traditional oriental medicine and
possesses a number of physiological activities. In this study, we used cell-based herbal extract
screening to identify longan fruit extract (LFE) as an activator of osteoblast differentiation.
LFE up-regulated alkaline phosphatase (ALP) activity, induced mineralization, and activated
Runx2 gene expression in MC3T3-E1 cells. Furthermore, treatment of MC3T3-E1 cells with LFE
promoted the phosphorylation of extracellular signal-regulated kinase1/2 (Erk1/2); however,
abrogation of Erk1/2 activation with PD98059 resulted in down-regulation of the phospho-
SMAD1/5/8 and Runx2 levels, which in turn reduced the ALP activity. Our findings suggest
that LFE exerts its osteogenic activity through activation of the ERK signaling pathway and
may have potential as an herbal therapeutic or a preventive agent for the treatment of
|Keywords||Longan fruit extract, osteoblast differentiation, Erk1/2 pathway, herbal medicine|
Apriyanto DR, Aoki C, Hartati S, Hanafi M, Kardono LB, Arsianti A, et al. 2015. Anti-hepatitis C virus activity of a crude extract from logan (Dimocarpus longan Lour.) leaves. Jpn. J. Infect Dis. [Epub ahead of print].
Bokui N, Otani T, Iqarashi K, Kaku J, Oda M, Nagaoka T, et al. 2008. Involvemnt of MAPK signaling molecules and Runx2 in the NELL1-induced osteoblastic differentiation. FEBS Lett. 582: 365-371.
Brude re r M, Richards RG, Alini M, S toddart MJ. 2 014. Role and regulation of RUNX2 in osteogenesis. Eur. Cell Mater. 28: 269-286.
Ge C, Xiao G, Jiang D, Yang Q, Hatch NE, Roca H, Franceschi RT. 2009. Identification and functional characterization of ERK/MAPK phosphorylation sites in the Runx2 transcription factor. J. Biol. Chem. 284: 32533-32543.
Huang RL, Yuan Y, Tu J, Zou GM, Li Q. 2014. Opposing TNF-α/IL-1β- and BMP-2-activated MAPK signaling pathways converge on Runx2 to regulate BMP-2-induced osteoblastic differentiation. Cell Death Dis. 5: e1187.
Lin CC, Chung YC, Hsu CP. 2012. Potential roles of longan flower and seed extracts for anti-cancer. World J. Exp. Med. 2: 78-85.
Fitzpatrick LA. 2002. Secondary causes of osteoporosis. Mayo Clin. Proc. 77: 453-468.
Marie PJ, Kassem M. 2011. Osteoblasts in osteoporosis: past, emerging, and future anabolic targets. Eur. J. Endocrinol. 165: 1-10.
Park S, Gwak J, Cho M, Song T, Won J, Kim DE, et al. 2006. Hexachlorophene inhibits Wnt/β-catenin pathway by promoting Siah-mediated β-catenin degradation. Mol. Pharmacol. 70:960-966.
Rangkadilok N, Tongchusak S, Boonhok R, Chaiyaroj SC, Junyaprasert VB, Buajeeb W, et al. 2012. In vitro antifungal activities of longan (Dimocarpus longan Lour.) seed extract. Fitoterapia 83: 545-553.
Re di IR. 2 002. P harmacothe arpy o f oste poorosis i n postmenopausal women: focus on safety. Expert Opin. Drug Saf. 1: 93-107.
Rodan GA. 1998. Bone homeostasis. Proc. Natl. Acad. Sci. USA 95: 13361-13362.
Tashjian AH Jr, Gagel RF. 2006. Teriparatide [human PTH(134)]: 2.5 ye ars of e xpe rie nce on the use and s afe ty o f the drug for the treatment of osteoporosis. J. Bone Miner. Res. 21: 354-365.
Tseng HC, Wu WT, Huang HS, Wu MC. 2014. Antimicrobial activities of various fractions of longan (Dimocarpus longan Lour. Fen Ke) seed extract. Int. J. Food Sci. Nutr. 65: 589-593.
Yang C, He N, Ling X, Ye M, Zhang C, Shao W, et al. 2008. The isolation and characterization of polysaccharides from longan pulp. Sep. Purif. Technol. 63: 226-230.
Zhang W, Guo H, Jing H, Li Y, Wang X, Zhang H, et al. 2014. Lactoferrin stimulates osteoblast differentiation through PKA and p38 pathways independent of lactoferrin’s receptor LRP1. J. Bone Miner. Res. 29: 1232-1243.
Zhou H, Choong P, McCarthy R, Chou ST, Martin TJ, Ng KW. 1994. In situ hybridization to show sequential expression of osteoblast gene markers during bone formation in vivo. J. Bone Miner. Res. 9: 1489-1499.