2015 ; 25(8):
|Author||Hyun Sung Lee, Jong Gyu Lim, Kook Han, Younghoon Lee, Sang Ho Choi|
|Affiliation||Korea Food Research Institute, Seongnam 463-746, Republic of Korea|
|Title||Molecular Analysis of Promoter and Intergenic Region Attenuator of the Vibrio vulnificus prx1ahpF Operon|
J. Microbiol. Biotechnol.2015 ; 25(8):
|Abstract||Prx1, an AhpF-dependent 2-Cys peroxiredoxin (Prx), was previously identified in Vibrio
vulnificus, a facultative aerobic pathogen. In the present study, transcription of the V. vulnificus
prx1ahpF genes, which are adjacently located on the chromosome, was evaluated by analyzing
the promoter and intergenic region of the two genes. Northern blot analyses revealed that
transcription of prx1ahpF results in two transcripts, the prx1 and prx1ahpF transcripts. Primer
extension analysis and a point mutational analysis of the promoter region showed that the two
transcripts are generated from a single promoter. In addition, the 3’ end of the prx1 transcript
at the prx1ahpF intergenic region was determined by a 3’RACE assay. These results suggested
that the prx1ahpF genes are transcribed as an operon, and the prx1 transcript was produced by
transcriptional termination in the intergenic region. RNA secondary structure prediction of
the prx1ahpF intergenic region singled out a stem-loop structure without poly(U) tract, and a
deletion analysis of the intergenic region showed that the atypical stem-loop structure acts as
the transcriptional attenuator to result in the prx1 and prx1ahpF transcripts. The combined
results demonstrate that the differential expression of prx1 and ahpF is accomplished by the
cis-acting transcriptional attenuator located between the two genes and thereby leads to the
production of a high level of Prx1 and a low level of AhpF.|
|Keywords||Vibrio vulnificus, peroxiredoxin, alkyl hydroperoxide reductase, transcriptional attenuation|
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