2015 ; 25(1):
|Author||Sooyeon Lim, Dong-Hoon Lee, Woori Kwak, Hakdong Shin, Hye-Jin Ku, Jong-eun Lee, Gun Eui Lee, Heebal Kim, Sang-Ho Choi, Sangryeol Ryu, Ju-Hoon Lee|
|Affiliation||Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea|
|Title||Comparative Genomic Analysis of Staphylococcus aureus FORC_001 and S. aureus MRSA252 Reveals the Characteristics of Antibiotic Resistance and Virulence Factors for Human Infection|
J. Microbiol. Biotechnol.2015 ; 25(1):
|Abstract||Staphylococcus aureus is an important foodborne pathogen that causes diverse diseases ranging
from minor infections to life-threatening conditions in humans and animals. To further
understand its pathogenesis, the genome of the strain S. aureus FORC_001 was isolated from a
contaminated food. Its genome consists of 2,886,017 bp double-stranded DNA with a GC
content of 32.8%. It is predicted to contain 2,728 open reading frames, 57 tRNAs, and 6 rRNA
operons, including 1 additional 5S rRNA gene. Comparative phylogenetic tree analysis of 40
complete S. aureus genome sequences using average nucleotide identity (ANI) revealed that
strain FORC_001 belonged to Group I. The closest phylogenetic match was S. aureus
MRSA252, according to a whole-genome ANI (99.87%), suggesting that they might share a
common ancestor. Comparative genome analysis of FORC_001 and MRSA252 revealed two
non-homologous regions: Regions I and II. The presence of various antibiotic resistance genes,
including the SCCmec cluster in Region I of MRSA252, suggests that this strain might have
acquired the SCCmec cluster to adapt to specific environments containing methicillin. Region
II of both genomes contains prophage regions but their DNA sequence identity is very low,
suggesting that the prophages might differ. This is the first report of the complete genome
sequence of S. aureus isolated from a real foodborne outbreak in South Korea. This report
would be helpful to extend our understanding about the genome, general characteristics, and
virulence factors of S. aureus for further studies of pathogenesis, rapid detection, and
epidemiological investigation in foodborne outbreak.|
|Keywords||Staphylococcus aureus, genome, foodborne pathogen, antibiotic resistance, virulence factor|
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