2014 ; 24(12):
|Author||Jae-Hyung Ahn, Woo-Suk Jeong, Min-Young Choi, Byung-Yong Kim, Jaekyeong Song, Hang-Yeon Weon|
|Affiliation||Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju, 565-851, Republic of Korea|
|Title||Phylogenetic Diversity of Dominant Bacterial and Archaeal Communities in Plant-Microbial Fuel Cells Using Rice Plants|
J. Microbiol. Biotechnol.2014 ; 24(12):
|Abstract||In this study, the phylogenetic diversities of bacterial and archaeal communities in a plantmicrobial
fuel cell (P-MFC) were investigated together with the environmental parameters,
affecting its performance by using rice as a model plant. The beneficial effect of the plant
appeared only during a certain period of the rice-growing season, at which point the
maximum power density was approximately 3-fold higher with rice plants. The temperature,
electrical conductivity (EC), and pH in the cathodic and anodic compartments changed
considerably during the rice-growing season, and a higher temperature, reduced difference in
pH between the cathodic and anodic compartments, and higher EC were advantageous to the
performance of the P-MFC. A 16S rRNA pyrosequencing analysis showed that the 16S rRNAs
of Deltaproteobacteria and those of Gammaproteobacteria were enriched on the anodes and
the cathodes, respectively, when the electrical circuit was connected. At the species level, the
operational taxonomic units (OTUs) related to Rhizobiales, Geobacter, Myxococcus, Deferrisoma,
and Desulfobulbus were enriched on the anodes, while an OTU related to Acidiferrobacter
thiooxydans occupied the highest proportion on the cathodes and occurred only when the
circuit was connected. Furthermore, the connection of the electrical circuit decreased the
abundance of 16S rRNAs of acetotrophic methanogens and increased that of
hydrogenotrophic methanogens. The control of these physicochemical and microbiological
factors is expected to be able to improve the performance of P-MFCs.|
|Keywords||plant-microbial fuel cell, bacterial community, archaeal community, 16S rRNA|
Ahn JH, Choi MY, Kim BY, Lee JS, Song J, Kim GY, Weon HY. 2014. Effects of water-saving irrigation on emissions of greenhouse gases and prokaryotic communities in rice paddy soil. Microb. Ecol. 68: 271-283.
Aklujkar M, Young N, Holmes D, Chavan M, Risso C, Kiss H, et al. 2010. The genome of Geobacter bemidjiensis, exemplar for the subsurface clade of Geobacter species that predominate in Fe(III)-reducing subsurface environments. BMC Genomics. 11: 490.
Arends JA, Blondeel E, Tennison S, Boon N, Verstraete W. 2012. Suitability of granular carbon as an anode material for sediment microbial fuel cells. J. Soils Sediments 12: 1197-1206.
Arends JA, Speeckaert J, Blondeel E, De Vrieze J, Boeckx P, Verstraete W, et al. 2014. Greenhouse gas emissions from rice microcosms amended with a plant microbial fuel cell. Appl. Microbiol. Biotechnol. 98: 3205-3217.
Bombelli P, Iyer D, Covshoff S, McCormick A, Yunus K, Hibberd J, et al. 2013. Comparison of power output by rice (Oryza sativa) and an associated weed (Echinochloa glabrescens) in vascular plant bio-photovoltaic (VP-BPV) systems. Appl. Microbiol. Biotechnol. 97: 429-438.
Bond DR, Holmes DE, Tender LM, Lovley DR. 2002. Electrode-reducing microorganisms that harvest energy from marine sediments. Science 295: 483-485.
Carbajosa S, Malki M, Caillard R, Lopez MF, Palomares FJ, Martín-Gago JA, et al. 2010. Electrochemical growth of Acidithiobacillus ferrooxidans on a graphite electrode for obtaining a biocathode for direct electrocatalytic reduction of oxygen. Biosens. Bioelectron. 26: 877-880.
Chiranjeevi P, Chandra R, Mohan SV. 2013. Ecologically engineered submerged and emergent macrophyte based system: an integrated eco-electrogenic design for harnessing power with simultaneous wastewater treatment. Ecol. Eng. 51: 181-190.
Chiranjeevi P, Mohanakrishna G, Venkata Mohan S. 2012. Rhizosphere mediated electrogenesis with the function of anode placement for harnessing bioenergy through CO2 sequestration. Bioresour. Technol. 124: 364-370.
Chun J, Kim K, Lee JH, Choi Y. 2010. The analysis of oral microbial communities of wild-type and Toll-like receptor 2deficient mice using a 454 GS FLX titanium pyrosequencer. BMC Microbiol. 10: 101.
Conrad R. 2007. Microbial ecology of methanogens and methanotrophs. Adv. Agron. 96: 1-63.
De Schamphelaire L, Boeckx P, Verstraete W. 2010. Evaluation of biocathodes in freshwater and brackish sediment microbial fuel cells. Appl. Microbiol. Biotechnol. 87: 1675-1687.
De Schamphelaire L, Bossche LVd, Dang HS, Höfte M, Boon N, Rabaey K, Verstraete W. 2008. Microbial fuel cells generating electricity from rhizodeposits of rice plants. Environ. Sci. Technol. 42: 3053-3058.
De Schamphelaire L, Cabezas A, Marzorati M, Friedrich MW, Boon N, Verstraete W. 2010. Microbial community analysis of anodes from sediment microbial fuel cells powered by rhizodeposits of living rice plants. Appl. Environ. Microbiol. 76: 2002-2008.
De Schamphelaire L, Rabaey K, Boeckx P, Boon N, Verstraete W. 2008. Outlook for benefits of sediment microbial fuel cells with two bio-electrodes. Microb. Biotechnol. 1: 446-462.
Deng H, Chen Z, Zhao F. 2012. Energy from plants and microorganisms: progress in plant–microbial fuel cells. ChemSusChem. 5: 1006-1011.
Dridi B, Fardeau M-L, Ollivier B, Raoult D, Drancourt M. 2012. Methanomassiliicoccus luminyensis gen. nov., sp. nov., a methanogenic archaeon isolated from human faeces. Int. J. Syst. Evol. Microbiol. 62: 1902-1907.
Franks AE, Nevin KP, Jia H, Izallalen M, Woodard TL, Lovley DR. 2009. Novel strategy for three-dimensional realtime imaging of microbial fuel cell communities: monitoring the inhibitory effects of proton accumulation within the anode biofilm. Energ. Environ. Sci. 2: 113-119.
Gil G -C, Chang I-S, K im BH, K im M , Jang J -K , Park H S, Kim HJ. 2003. Operational parameters affecting the performance of a mediator-less microbial fuel cell. Biosens. Bioelectron. 18:327-334.
Hallberg K, Hedrich S, Johnson DB. 2011. Acidiferrobacter thiooxydans, gen. nov. sp. nov.; an acidophilic, thermotolerant, facultatively anaerobic iron- and sulfur-oxidizer of the family Ectothiorhodospiraceae. Extremophiles 15: 271-279.
He Z, Angenent LT. 2006. Application of bacterial biocathodes in microbial fuel cells. Electroanalysis 18: 2009-2015.
Helder M, Strik D, Hamelers H, Buisman C. 2012. The flatplate plant-microbial fuel cell: the effect of a new design on internal resistances. Biotechnol. Biofuels 5: 1-11.
Holmes DE, Bond DR, Lovley DR. 2004. Electron transfer by Desulfobulbus propionicus to Fe(III) and graphite electrodes. Appl. Environ. Microbiol. 70: 1234-1237.
Holmes DE, Bond DR, O’Neil RA, Reimers CE, Tender LR, Lovley DR. 2004. Microbial communities associated with electrodes harvesting electricity from a variety of aquatic sediments. Microb. Ecol. 48: 178-190.
Holmes DE, O'Neil RA, Vrionis HA, N'Guessan LA, OrtizBernad I, Larrahondo MJ, et al. 2007. Subsurface clade of Geobacteraceae that predominates in a diversity of Fe(III)reducing subsurface environments. ISME J. 1: 663-677.
Hong SW, Chang IS, Choi YS, Chung TH. 2009. Experimental evaluation of influential factors for electricity harvesting from sediment using microbial fuel cell. Bioresour. Technol. 100: 3029-3035.
Hur M, Kim Y, Song HR, Kim JM, Choi YI, Yi H. 2011. Effect of genetically modified poplars on soil microbial communities during the phytoremediation of waste mine tailings. Appl. Environ. Microbiol. 77: 7611-7619.
Ishii Si, Hotta Y, Watanabe K. 2008. Methanogenesis versus electrogenesis: morphological and phylogenetic comparisons of microbial communities. Biosci. Biotechnol. Biochem. 72: 286-294.
Kaku N, Yonezawa N, Kodama Y, Watanabe K. 2008. Plant/microbe cooperation for electricity generation in a rice paddy field. Appl. Microbiol. Biotechnol. 79: 43-49.
Kato S, Hashimoto K, Watanabe K. 2012. Microbial interspecies electron transfer via electric currents through conductive minerals. Proc. Natl. Acad. Sci. USA 109: 10042-10046.
K endall M M, W ardlaw G D, Tang CF, Bonin A S, L iu Y , Valentine DL. 2007. Diversity of Archaea in marine sediments from Skan Bay, Alaska, including cultivated methanogens, and description of Methanogenium boonei sp. nov. Appl. Environ. Microbiol. 73: 407-414.
Kiely PD, Regan JM, Logan BE. 2011. The electric picnic:synergistic requirements for exoelectrogenic microbial communities. Curr. Opin. Biotechnol. 22: 378-385.
Kodama Y, Watanabe K. 2011. Rhizomicrobium electricum sp. nov., a facultatively anaerobic, fermentative, prosthecate bacterium isolated from a cellulose-fed microbial fuel cell. Int. J. Syst. Evol. Microbiol. 61: 1781-1785.
Kouzuma A, Kasai T, Nakagawa G, Yamamuro A, Abe T, Watanabe K. 2013. Comparative metagenomics of anodeassociated microbiomes developed in rice paddy-field microbial fuel cells. PLoS One 8: e77443.
Kuever J, Rainey FA, Widdel F. 2005. Family II. Desulfobulbaceae fam. nov., pp. 988-999. In B renner D J, K rieg N R, G arrity GM, Staley JT, Boone DR, Vos P, et al. (eds.). Bergey's Manual of Systematic Bacteriology. Springer, New York.
Liu H, Cheng S, Logan BE. 2005. Power generation in fedbatch microbial fuel cells as a function of ionic strength, temperature, and reactor configuration. Environ. Sci. Technol. 39: 5488-5493.
Liu S, Song H, Li X, Yang F. 2013. Power generation enhancement by utilizing plant photosynthate in microbial fuel cell coupled constructed wetland system. Int. J. Photoenergy 2013: 10.
Liu S, Song H, Wei S, Yang F, Li X. 2014. Bio-cathode materials evaluation and configuration optimization for power output of vertical subsurface flow constructed wetland —Microbial fuel cell systems. Bioresour. Technol. 166: 575-583.
Logan BE. 2009. Exoelectrogenic bacteria that power microbial fuel cells. Nat. Rev. Microbiol. 7: 375-381.
Logan BE, Hamelers B, Rozendal R, Schröder U, Keller J, Freguia S, et al. 2006. Microbial fuel cells: methodology and technology. Environ. Sci. Technol. 40: 5181-5192.
Logan BE, Regan JM. 2006. Electricity-producing bacterial communities in microbial fuel cells. Trends Microbiol. 14:512-518.
Nakasono S, Matsumoto N, Saiki H. 1997. Electrochemical cultivation of Thiobacillus ferrooxidans by potential control. Bioelectrochem. Bioener. 43: 61-66.
Popat SC, Ki D, Rittmann BE, Torres CI. 2012. Importance of OH transport from cathodes in microbial fuel cells. ChemSusChem 5: 1071-1079.
Rozendal RA, Hamelers HVM, Buisman CJN. 2006. Effects of membrane cation transport on pH and microbial fuel cell performance. Environ. Sci. Technol. 40: 5206-5211.
Sanford RA, Cole JR, Tiedje JM. 2002. Characterization and description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an aryl-halorespiring facultative anaerobic Myxobacterium. Appl. Environ. Microbiol. 68: 893-900.
Schamphelaire LD, Bossche LVd, Dang HS, Höfte M, Boon N, Rabaey K, Verstraete W. 2008. Microbial fuel cells generating electricity from rhizodeposits of rice plants. Environ. Sci. Technol. 42: 3053-3058.
Shehab N, Li D, Amy G, Logan B, Saikaly P. 2013. Characterization of bacterial and archaeal communities in air-cathode microbial fuel cells, open circuit and sealed-off reactors. Appl. Microbiol. Biotechnol. 97: 9885-9895.
Slobodkina GB, Reysenbach A-L, Panteleeva AN, Kostrikina NA, Wagner ID, Bonch-Osmolovskaya EA, Slobodkin AI. 2012. Deferrisoma camini gen. nov., sp. nov., a moderately thermophilic, dissimilatory iron(III)-reducing bacterium from a deep-sea hydrothermal vent that forms a distinct phylogenetic branch in the Deltaproteobacteria. Int. J. Syst. Evol. Microbiol. 62: 2463-2468.
Strik DPBTB, Hamelers HVM, Snel JFH, Buisman CJN. 2008. Green electricity production with living plants and bacteria in a fuel cell. Int. J. Energ. Res. 32: 870-876.
Strik DPBTB, Picot M, Buisman CJN, Barrière F. 2013. pH and temperature determine performance of oxygen reducing biocathodes. Electroanalysis 25: 652-655.
Strik DPBTB, Timmers RA, Helder M, Steinbusch KJJ, Hamelers HVM, Buisman CJN. 2011. Microbial solar cells:applying photosynthetic and electrochemically active organisms. Trends Biotechnol. 29: 41-49.
Sun Y, Wei J, Liang P, Huang X. 2012. Microbial community analysis in biocathode microbial fuel cells packed with different materials. AMB Express 2: 21.
Timmers RA, Strik DPBTB, Hamelers HVM, Buisman CJN. 2013. Electricity generation by a novel design tubular plant microbial fuel cell. Biomass Bioenergy 51: 60-67.
Torres CI, Kato Marcus A, Rittmann BE. 2008. Proton transport inside the biofilm limits electrical current generation by anode-respiring bacteria. Biotechnol. Bioeng. 100: 872-881.
Xia X, Sun Y, Liang P, Huang X. 2012. Long-term effect of set potential on biocathodes in microbial fuel cells: electrochemical and phylogenetic characterization. Bioresour. Technol. 120:26-33.
Zhang Y, Sun J, Hu Y, Li S, Xu Q. 2012. Bio-cathode materials evaluation in microbial fuel cells: a comparison of graphite felt, carbon paper and stainless steel mesh materials. Int. J. Hydrogen Energy 37: 16935-16942.