2015 ; 25(5):
|Author||Biswajit Podder, Woong Sik Jang, Kung-Woo Nam, Byung-Eui Lee, Ho-Yeon Song|
|Affiliation||Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan 330-090, Republic of Korea|
|Title||Ursolic Acid Activates Intracellular Killing Effect of Macrophages During Mycobacterium tuberculosis Infection|
J. Microbiol. Biotechnol.2015 ; 25(5):
|Abstract||Tuberculosis is one of the most threatening infectious diseases to public health all over the
world, for which Mycobacterium tuberculosis (MTB) is the etiological agent of pathogenesis.
Ursolic acid (UA) has immunomodulatory function and exhibits antimycobacterial activity.
However, the intracellular killing effect of UA has yet to be elucidated. The aim of this study
was to evaluate the intracellular killing effect of UA during mycobacterial infection. The
intracellular killing activity of UA was evaluated in the macrophage cell line THP-1 by the
MGIT 960 system as well as by CFU count. The production of reactive oxygen species (ROS)
and the level of nitric oxide (NO) were measured using DCF-DA and Griess reagent,
respectively. Phagocytosis was observed by a fluorescence-based staining method, and the
colony forming units were enumerated on 7H11 agar medium following infection. In addition,
MRP8 mRNA expression was measured by qRT-PCR. UA significantly decreased the number
of intracellular Mycobacterium through generation of ROS and NO. In addition, it profoundly
activated the phagocytosis process of THP-1 cells during MTB-infection. Furthermore, our
data demonstrated that UA activated the phagocytosis process in human monocyte cells
through MRP8 induction. These data suggest that UA firmly contributes to the intracellular
killing effect of macrophages during mycobacterial infection.|
|Keywords||Mycobacterial, Ursolic acid, Macrophages, Intracellular, Phagocytosis|
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