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Research articleMolecular and Cellular Microbiology (MCM) | Biochemistry and Physiology
Croton hirtus L'Hér Extract Prevents Inflammation in RAW264.7 Macrophages Via Inhibition of NF-κB Signaling Pathway
1School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, 2Ministry of Health, Institute of Traditional Medicine, Vientiane 116, Lao PDR, 3International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea, 4Institute of Agricultural Science & Technology, Kyungpook National University, Daegu 41566, Republic of KoreaReceived: August 23, 2019; Accepted: November 11, 2019
J. Microbiol. Biotechnol. 2020; 30(4): 490-496
Published April 28, 2020
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractConsumption of anti-inflammatory nutraceuticals may help treat or prevent inflammation-related illnesses such as diabetes, cardiovascular disease, and cancer. This study evaluated the effect of Croton hirtus L'Hér extract (CHE) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor kappa-B (NF-κB) signaling cascades. CHE significantly suppressed LPS-induced NO production and inducible nitric oxide synthase (iNOS) expression in RAW264.7 macrophages, although cyclooxygenase (COX)-2 expression was not affected. CHE also suppressed LPS-induced IκB kinase (IKK), IκB, and p65 phosphorylation in RAW264.7 cells. Western blot and immunofluorescence assays of cytosol and nuclear p65 and the catalytic subunit of NF-κB showed that CHE suppressed LPS-induced p65 translocation from the cytosol to the nucleus. CHE also suppressed LPS-induced Interleukin (IL)-6 and tumor necrosis factor (TNF)-α production in RAW264.7 cells. These results suggest that CHE prevents NO-mediated inflammation by suppressing NF-κB and inflammatory cytokines..
KeywordsCroton hirtus L, inflammation, NF-&kappa,B, nutraceuticals, macrophage, nitric oxide