Bioprospecting of Novel and Bioactive Metabolites from Endophytic Fungi Isolated from Rubber Tree Ficus elastica Leaves

Zhuang Ding; Tao Tao; Lili Wang; Yanna Zhao; Huiming Huang; Demeng Zhang; Min Liu; Zhengping Wang  and Jun Han

doi:10.4014/jmb.1901.01015

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Food Microbiology and Biotechnology(FMB) | Bioactive compounds and Physiological properties

Bioprospecting of Novel and Bioactive Metabolites from Endophytic Fungi Isolated from Rubber Tree Ficus elastica Leaves

Zhuang Ding ^1*, Tao Tao ¹, Lili Wang ¹, Yanna Zhao ¹, Huiming Huang ², Demeng Zhang ³, Min Liu ¹, Zhengping Wang ¹ and Jun Han ¹

¹Institute of BioPharmaceutical Research, Liaocheng University,P.R China, ²School of Life Sciences, Liaocheng University, P.R China, ³State key laboratory of bioactive seaweed substances, Qingdao brightmoon seaweed Group Co Ltd,PR China

Correspondence to:

Zhuang Ding
dingzhuang_2010@163.com

Received: January 8, 2019; Accepted: April 19, 2019

J. Microbiol. Biotechnol. 2019; 29(5): 731-738

Published May 28, 2019 https://doi.org/10.4014/jmb.1901.01015

Abstract

Endophytic fungi are an important component of plant microbiota, and have the excellent capacity for producing a broad variety of bioactive metabolites. These bioactive metabolites not only affect the survival of the host plant, but also provide valuable lead compounds for novel drug discovery. In this study, forty-two endophytic filamentous fungi were isolated from Ficus elastica leaves, and further identified as seven individual taxa by ITS-rDNA sequencing. The antimicrobial activity of these endophytic fungi was evaluated against five pathogenic microorganisms. Two strains, Fes1711 (Penicillium funiculosum) and Fes1712 (Trichoderma harzianum), displayed broad-spectrum bioactivities. Our following study emphasizes the isolation, identification and bioactivity testing of chemical metabolites produced by T. harzianum Fes1712. Two new isocoumarin derivatives (1 and 2), together with three known compounds (3–5) were isolated, and their structures were elucidated using NMR and MS. Compounds 1 and 2 exhibited inhibitory activity against Escherichia coli. Our findings reveal that endophytic fungi from the rubber tree F. elastica leaves exhibit unique characteristics and are potential producers of novel natural bioactive products.

Keywords

Antimicrobial activity, endophytic fungi, Trichoderma harzianum, isocoumarin, metabolites, ficus elastica

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