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Research articleFood Biotechnology (FB) | Bioactive compounds and Physiological properties
Genetic and functional analysis of the DKxanthene biosynthetic gene cluster from Myxococcusstipitatus DSM 14675
1Department of Biotechnology, Hoseo University, Asan 31499, Republic of Korea, 2Biocenter,Gyeonggido Business & Science Accelerator (GBSA), Suwon 16229, Republic of KoreaReceived: February 26, 2018; Accepted: May 29, 2018
J. Microbiol. Biotechnol. 2018; 28(7): 1068-1077
Published July 28, 2018
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractDKxanthenes area class of yellow secondary metabolites produced by myxobacterial genera Myxococcusand Stigmatella. We identified a putative 49.5 kb DKxanthene biosynthetic gene cluster from M. stipitatus DSM 14675 by genomic sequence and mutational analysis. The cluster was comprisedof 15 genes (MYSTI_06004-MYSTI_06018) encoding polyketide synthases, non-ribosomal peptide synthases, and proteins with unknown functions. Disruption of the genes by plasmid insertion resulted in defects in the production of yellow pigments. High-performance liquid chromatography and liquid chromatography-tandem mass spectrometryanalysis indicated that the yellow pigments produced by M. stipitatus DSM 14675 might be noble DKxanthene derivatives.M. stipitatus did not require DKxanthenes for the formation of heat-resistant viable spores,unlike M. xanthus. Furthermore, DKxanthenes showed growth inhibitory activity against the fungi Aspergillusniger, Candida albicans, and Rhizopusstolonifer.
KeywordsMyxobacteria, Myxococcus stipitatus, secondary metabolite, DKxanthene, biosynthetic gene