Related articles in JMB
Research article | 2017-03-28Sathiyaraj Srinivasan , Sangyong Lim , Jae-Hyun Lim , Hee-Young Jung and Myung Kyum KimResearch article | 2020-04-28Arxel G. Elnar , Min-Gon Kim , Ju-Eun Lee , Rae-Hee Han , Sung-Hee Yoon , Gi-Yong Lee , Soo-Jin Yang and Geun Bae KimResearch article | 2016-08-28
Research articleEnvironmental Microbiology and Biotechnology (EMB) | Microbial Ecology and Taxonomy
Deinococcus rubrus sp. nov., a Bacterium Isolated from Antarctic Coastal Sea Water
1Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 01797, Republic of Korea, 2Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, 3Marine Environment Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea, 4College of Agricultural and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea, 5Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaReceived: September 5, 2016; Accepted: December 6, 2016
J. Microbiol. Biotechnol. 2017; 27(3): 535-541
Published March 28, 2017
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractTwo Gram-staining-negative, red-pinkish, coccus-shaped, non-motile, and aerobic bacterial strains, designated Ant21T and Ant22, were isolated from the Antarctic coastal sea water. Strains Ant21T and Ant22 showed UVC and gamma radiation resistance. Phylogenetic analyses based on 16S rRNA gene sequences determined that these strains belong to the genus Deinococcus. Through the analyses of the 16S rRNA gene sequences, strains Ant21T and Ant22 were found to have 97.7% and 97.8% similarity to Deinococcus marmoris DSM 12784T and 97.0% and 97.2% similarity to Deinococcus saxicola AA-1444T, respectively. The sequence similarity with the type strains of other Deinococcus species was less than 96.9% for both strains. Strains Ant21T and Ant22 shared relatively high 16S rRNA gene sequence similarity (99.3%) and had a closely related DNA reassociation value of 84 ± 0.5%. Meanwhile, they showed a low level of DNA-DNA hybridization (<30%) with other closely related species of the genus Deinococcus. The two strains also showed typical chemotaxonomic features for the genus Deinococcus, in terms of the major polar lipid (phosphoglycolipid) and the major fatty acids (C16:0, C16:1 ω6c/ ω7c, iso-C17:0 , and iso-C15:0). They grew at temperatures between 4°C and 30°C and at pH values of 6.0-8.0. Based on the physiological characteristics, the 16S rRNA gene sequence analysis results, and the low DNA-DNA reassociation level with Deionococcus marmoris, strains Ant21T (= KEMB 9004-167T =JCM 31436T) and Ant22 (KEMB 9004-168 =JCM 31437) represent novel species belonging to the genus Deinococcus, for which the name Deinococcus rubrus is proposed.
Keywordscoastal sea water, Deinococcus, radiation resistance, taxonomy