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Research articleMolecular and Cellular Microbiology (MCM) | Clinical Microbiology and Biomedical Sciences
Persistence of Multidrug-Resistant Acinetobacter baumannii Isolates Harboring blaOXA-23 and bap for 5 Years
1Department of Biomedical Laboratory Science, Far East University, Eumseong 27601, Republic of Korea, 2Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon 34134, Republic of KoreaReceived: April 18, 2016; Accepted: May 23, 2016
J. Microbiol. Biotechnol. 2016; 26(8): 1481-1489
Published August 28, 2016
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractThe emergence and dissemination of carbapenemase-producing Acinetobacter baumannii isolates have been reported worldwide, and A. baumannii isolates harboring blaOXA-23 are often resistant to various antimicrobial agents. Antimicrobial resistance can be particularly strong for biofilm-forming A. baumannii isolates. We investigated the genetic basis for carbapenem resistance and biofilm-forming ability of multidrug-resistant (MDR) clinical isolates. Ninetytwo MDR A. baumannii isolates were collected from one university hospital located in the Chungcheong area of Korea over a 5-year period. Multiplex PCR and DNA sequencing were performed to characterize carbapenemase and bap genes. Clonal characteristics were analyzed using REP-PCR. In addition, imaging and quantification of biofilms were performed using a crystal violet assay. All 92 MDR A. baumannii isolates involved in our study contained the blaOXA-23 and bap genes. The average absorbance of biomass in Bap-producing strains was much greater than that in non-Bap-producing strains. In our study, only three REP-PCR types were found, and the isolates showing type A or type B were found more than 60 times among unique patients during the 5 years of surveillance. These results suggest that the isolates have persisted and colonized for 5 years, and biofilm formation ability has been responsible for their persistence and colonization.
Keywordscarbapenemase, Acinetobacter baumannii, antimicrobial resistance, biofilm, crystal violet assay