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Research articleFood Microbiology and Biotechnology
Exopolysaccharide-Overproducing Lactobacillus paracasei KB28 Induces Cytokines in Mouse Peritoneal Macrophages via Modulation of NF-κB and MAPKs
1Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Korea, 2Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-857, Korea, 3Department of Food Science and Technology, Chungbuk National University, Cheongju 361-763, KoreaReceived: May 16, 2011; Accepted: July 26, 2011
J. Microbiol. Biotechnol. 2011; 21(11): 1174-1178
Published November 28, 2011
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractExopolysaccharides (EPSs) are microbial polysaccharides that are released outside of the bacterial cell wall. There have been few studies on EPS-producing lactic acid bacteria that can enhance macrophage activity and the underlying signaling mechanism for cytokine expression. In the current study, EPS-overproducing Lactobacillus (L.) paracasei KB28 was isolated from kimchi and cultivated in conditioned media containing glucose, sucrose, and lactose. The whole bacterial cells were obtained with their EPS being attached, and the cytokine-inducing activities of these cells were investigated. Gas chromatography analysis showed the presence of glucose, galactose, mannose, xylose, arabinose, and rhamnose in EPS composition. EPS-producing L. paracasei KB28 induced the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-12 in mouse macrophages. This strain also caused the degradation of IκBα and phosphorylation of the major MAPKs: Jun N-terminal kinase (JNK), p38, and extracellular signal-regulated kinase (ERK)1/2. The use of pharmacological inhibitors showed that different signaling pathways were involved in the induction of TNF-α, IL-6 and IL-12 by L. paracasei KB28. Our results provide information for a better understanding of molecular mechanisms of the immunomodulatory effect of foodderived EPS-producing lactic acid bacteria.
Keywordsexopolysaccharides, Lactobacillus paracasei, macrophage, cytokine, signaling