Related articles in JMB
Identification of Genes Associated with Fumonisin Biosynthesis in Fusarium verticillioides via Proteomics and Quantitative Real-Time PCRResearch article | 2020-04-28
Biokinetics of protein degrading Clostridium cadaveris and Clostridium sporogenes in batch and continuous mode of operationsResearch article | 2019-11-28Microbial Cell Biology
Identification of Genes Associated with Fumonisin Biosynthesis in Fusarium verticillioides via Proteomics and Quantitative Real-Time PCR
Department of Plant Pathology and Microbiology, Program for the Biology of Filamentous Fungi, Texas A&M University, CollegeStation, TX 77843, U.S.A.
J. Microbiol. Biotechnol. 2008; 18(4): 648-657
Published April 28, 2008
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractIn this study, we used functional genomic strategies, proteomics and quantitative real-time (qRT)-PCR, to advance our understanding of genes associated with fumonisin production in the fungus Fusarium verticillioides. Earlier studies have demonstrated that deletion of the FCC1 gene, which encodes a C-type cyclin, leads to a drastic reduction in fumonisin production and conidiation in the mutant strain (FT536). The premise of our research was that comparative analysis of F. verticillioides wild-type and FT536 proteomes will reveal putative proteins, and ultimately corresponding genes, that are important for fumonisin biosynthesis. We isolated proteins that were significantly upregulated in either the wild type or FT536 via two-dimensional polyacrylamide gel electrophoresis, and subsequently obtained sequences by mass spectrometry. Homologs of identified proteins, e.g., carboxypeptidase, laccase, and nitrogen metabolite repression protein, are known to have functions involved in fungal secondary metabolism and development. We also identified gene sequences corresponding to the selected proteins and investigated their transcriptional profiles via quantitative real-time (qRT)-PCR in order to identify genes that show concomitant expression patterns during fumonisin biosynthesis. These genes can be selected as targets for functional analysis to further verify their roles in
KeywordsFumonisin regulation, fungal development, proteomics, quantitative real-time PCR, gene discovery