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Research article

Food Microbiology and Biotechnology(FMB)  |  Foodborne Pathogens and Food Safety

Prevalence and Genetic Characterization of mcr-1-Positive Escherichia coli Isolated from Retail Meats in South Korea

Seokhwan Kim1,2†, Hansol Kim1†, Hai-Seong Kang1, Yonghoon Kim1, Migyeong Kim1, Hyosun Kwak1*, and Sangryeol Ryu2*

1Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of Korea
2Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea

Correspondence to:
H.Kwak
Phone: +82-43-719-4301
Fax: +82-43-719-4300
E-mail: hyoskwak@korea.kr
S.Ryu
Phone: +82-2-880-4856
Fax: +82-2-873-5095
E-mail: sangryu@snu.ac.kr

†These authors contributed equally to this work.
Received: July 6, 2020; Revised: August 31, 2020; Accepted: September 18, 2020

J. Microbiol. Biotechnol. 2020; 30(12): 1862-1869

Published December 28, 2020 https://doi.org/10.4014/jmb.2007.07008

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

The spread of plasmid-mediated colistin resistance has posed a serious threat to public health owing to its effects on the emergence of pandrug-resistant bacteria. In this study, we investigated the prevalence and characteristics of mcr-1-positive Escherichia coli isolated from retail meat samples in Korea. In total, 1,205 E. coli strains were isolated from 3,234 retail meat samples in Korea. All E. coli strains were subjected to antimicrobial susceptibility testing and were examined for the presence of mcr-1 gene. All mcr-1-positive E. coli (n = 10, 0.8%) from retail meat were subjected to pulse-field gel electrophoresis (PFGE) and whole-genome sequencing (WGS). The transferability of mcr-1 gene was determined by conjugation assays. The mcr-1-positive strains exhibited diverse clonal types. Our mcr-1 genes were located in plasmids belonged to the IncI2 (n = 1) and IncX4 (n = 8) types, which were reported to be prevalent in Asia and worldwide, respectively. Most mcr-1 genes from mcr-1-positive strains (9/10) were transferable to the recipient strain and the transfer frequencies ranged from 2.4 × 10-3 to 9.8 × 10-6. Our data suggest that the specific types of plasmid may play an important role in spreading plasmid-mediated colistin resistance in Korea. Furthermore, our findings suggest that the retail meat may be an important tool for disseminating plasmid-mediated colistin resistance.

Keywords

Colistin resistance, mcr-1, prevalence, IncI2 plasmid, IncX4 plasmid, retail meats

Graphical Abstract


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