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Effect of Rosemary Essential Oil and Trichoderma koningiopsis VOCs on Pathogenic Fungi Responsible for Ginseng Root-rot DiseaseKhalid Abdallah Hussein 1, 2, Young Don Lee 1 and Jin Ho Joo 1*
1Soil Biochemistry Lab, Department of Biological Environment, Kangwon National University, Republic of Korea, 2Botany and Microbiology Department, Faculty of Science, Assiut University, 71516, Assiut, EgyptReceived: February 11, 2020; Accepted: April 6, 2020
J. Microbiol. Biotechnol.
Published online April 9, 2020
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractRosemary essential oil was evaluated for antifungal potentiality against six major ginseng pathogens Sclerotinia sclerotiorum, S. nivalis, Cylindrocarpon destructans, Alternaria panax, Botrytis cinerea, and Fusarium oxysporum. The in vitro fungicidal effects of two commonly used fungicides, namely mancozeb and fenhexamid and the volatile organic compounds (VOCs) of Trichoderma koningiopsis T-403 on the mycelial growth were investigated. The results showed that rosemary essential oil is active against all of the pathogenic strains of ginseng root rot, whereas, rosemary oil displayed a high ability to inhibit the Sclerotinia spp. growth. “The highest sensitivity was S. nivalis, with complete inhibition of growth at 0.1% v/v of rosemary oil, followed by Alternaria panax which exhibited 100 % inhibition at 0.3% v/v of the oil. Minimum inhibitory concentrations (MICs) of rosemary oil ranged from 0.1 % to 0.5 % (v/v). Chemical analysis using GC-MS showed the presence of thirty-two constituents within rosemary oil from R. officinals L. Camphore type is the most frequent sesquiterpene in rosemary oil composition. Mancozeb and fenhexamid showed their highest inhibition effect (45% and 30%, respectively) against A. panax. T. koningiopsis T-403 showed its highest inhibition effect (84%) against Cylindrocarpon destructans isolate. This study may hurry the application of antifungal natural substances from rosemary and Trichoderma in the prevention and control of phytopathogenic strains in ginseng root infections..
KeywordsGinseng-root rot, VOCs, essential oil, antifungal activity, rosemary