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Research articleMolecular and Cellular Microbiology / Biomedical Sciences | Biochemistry and Physiology
Smilax guianensis Vitman extract prevents LPS-induced inflammation by inhibiting the NF-κB pathway in RAW 264.7 cellsJu Gyeong Kim 1, Min Jeong Kim 1, Ji Su Lee 2, Kongmany Sydara 3, Sangwoo Lee 4, Sanguine Byun 5 and Sung Keun Jung 1, 6*
1School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, 2Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea, 3Ministry of Health, Institute of Traditional Medicine, Vientiane 116, Lao PDR, 4International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, 5Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea, 6Institute of Agricultural Science & Technology, Kyungpook National University, Daegu 41566, Republic of KoreaReceived: November 19, 2019; Accepted: March 22, 2020
J. Microbiol. Biotechnol.
Published online March 24, 2020
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractNutraceutical treatments can reduce inflammation and prevent the development of inflammatory diseases. In this study, the anti-inflammatory effects of Smilax guianensis Vitman extract (SGE) were examined. SGE suppressed lipopolysaccharide (LPS)-mediated nitrite production in RAW 264.7 cells. SGE also prevented the LPS-induced expression of inducible nitric oxide synthase (iNOS) but not cyclooxygenase (COX)-2. Western blot analysis showed that SGE attenuated LPS-induced phosphorylation of IκB kinase, inhibitor of kappa B (IκB), and p65. Additionally, SGE inhibited LPS-induced IκB degradation in RAW 264.7 cells. Western blot analysis of the cytosolic and nuclear fractions, as well as immunofluorescence assay results, revealed that SGE suppressed LPS-induced p65 nuclear translocation in RAW 264.7 cells. Moreover, SGE reduced LPS-induced interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) mRNA expression and IL-1β and IL-6 protein expression in RAW 264.7 cells. Collectively, these results indicate that SGE suppresses the NF-κB signaling pathway and thereby inhibits the production of NO, IL-1β, and IL-6.
KeywordsSmilax guianensis Vitman extract, nutraceuticals, nitric oxide, nitric oxide synthase, inflammation, NF-κB