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Research article

Molecular and Cellular Microbiology (MCM)  |  Pathogenesis and Host-Microbial interactions

Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice

Alisha Wehdnesday Bernardo Reyes 1, Son Hai Vu 1, Tran Xuan Ngoc Huy 1, Wongi Min 1, Hu Jang Lee 1, Hong Hee Chang 1, John Hwa Lee 2 and Suk Kim 1*

1Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea
2College of Veterinary Medicine, Chonbuk National University, Iksan, 54596, Republic of Korea

Received: November 18, 2019; Accepted: January 29, 2020

J. Microbiol. Biotechnol. 2020; 30(5): 642-648

Published May 28, 2020 https://doi.org/10.4014/jmb.1911.11037

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

In this study, we investigated the effects of linoleic acid (LA) treatment on Brucella abortus infection in professional phagocyte RAW264.7 cells, particularly during the pathogen’s invasion and intracellular growth in these cells, as well as in murine model BALB/c mice focusing on bacterial splenic proliferation and immunoregulatory activities. LA inhibited the growth of Brucella in a doseand time-dependent manner. The ability of the pathogen to enter the phagocytes was inhibited as was its survival within these cells. This was accompanied by increased nitrite accumulation in these cells at 24 h post-infection. The concentration of LA used in the present study did not affect the total body weight or liver function of the mice. During Brucella infection, the total splenic weight of these animals was not changed; rather, resistance to bacterial proliferation was enhanced in the spleen. Furthermore, mice treated with LA displayed elevated levels of IL-12 and IFN-γ but reduced levels of IL-10 during infection. The findings in this study showed the regulatory role of LA against B. abortus infection suggesting its potential use in designing intervention strategy for brucellosis.

Keywords

Brucella abortus, cytokines, invasion, linoleic acid, spleen weight

Graphical Abstract


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