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Research articleBioactive Compounds / Food Microbiology | Probiotics and Foodborne Microorganisms
Genome analysis of two Lactobacillus plantarum strains, LLY-606 and pc-26, for evaluating their potential as probioticsdun Su 1, 2, Lifeng Liang 1, Zhenzhen Li 2, 3, Qingnan Lin 2, Can Peng 1, 4, Xuejing Lei 1, 2 and Songling liu 1, 2*
BGI Institute of Applied Agriculture, BGI-Shenzhen, Shenzhen, 518120, China, BGI-Shenzhen, Shenzhen, 518083, China, BGI Sanshengyuan Co., Ltd, BGI-Shenzhen, Shenzhen, 518120, China, Shenzhen Key Laboratory of Bioenergy, BGI-Shenzhen, Shenzhen, 518083, ChinaReceived: July 4, 2019; Accepted: January 21, 2020
J. Microbiol. Biotechnol.
Published online January 23, 2020
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractLactobacillus plantarum was not only one of the most popular probiotics, but also one of the most versatile lactic acid bacteria. L. plantarum LLY-606 and L. plantarum pc-26 are strains isolated from human gut that are intended to be explored as probiotics. In this study, the genome sequences of LLY-606 and pc-26 were sequenced, and multiple genes related to probiotic properties were analyzed. First, the pathogenicity of these strains was evaluated, and antibiotic resistance genes were surveyed at the whole genome level to determine their primary safety. And then, genes for stress response, plantaricin (pln) biosynthesis, extracellular polysaccharide (EPS) biosynthesis, and bile salt hydrolase (BSH) were analyzed to evaluate their industrial utilization, adhesive capacity, and survival ability in gut, which were properties fundamental for probiotic strains. The physiological features assured by these genes were assayed in vitro. The strains were then evaluated in vivo for their ability to lower cholesterol, and they were both found to be effective in improving hypercholesterolemia in golden hamsters. In this study, a genetic pre-evaluation was conducted through genome analysis combined with in vitro physiological assay, and the probiotic properties of these strains were verified in vivo.
KeywordsLactobacillus plantarum, genomics, probiotic, cholesterol-lowering effect