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Environmental Microbiology and Biotechnology (EMB) | Microbial Ecology and Taxonomy
Acinetobacter pullorum sp. nov., Isolated from Chicken Meat
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea
Received: February 17, 2020; Accepted: March 16, 2020
J. Microbiol. Biotechnol. 2020; 30(4): 526-532
Published April 28, 2020
Copyright © The Korean Society for Microbiology and Biotechnology.
A bacterial strain, designated B301T, isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomy approach. Cells were Gram-stain-negative, non-motile, obligate-aerobic coccobacilli, catalase-positive, and oxidase-negative. The optimum growth conditions were 30°C, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that the strain B301T belonged to the genus Acinetobacter, with highest sequence similarities (97.12%) with A. celticus ANC 4603T and A. sichuanensis WCHAc060041T. The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95–96% and 70%, respectively). The DNA G+C content of strain B301T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C16:1 ω6c/C16:1 ω7c), C16:0, and C18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301T (=KACC 21653T = JCM 33942T).
Acinetobacter pullorum sp. nov., chicken meat, taxonomy, antimicrobial resistance
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