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Research articleMolecular and Cellular Microbiology (MCM) | Pathogenesis and Host-Microbial interactions
β-Sitosterol Contributes in the Resistance to Invasion and Survival of Brucella abortus 544 within RAW264.7 Cells, and Cytokine Production with Reduced Susceptibility to Infection in BALB/c MiceAlisha Wehdnesday Bernardo Reyes 1, Lauren Togonon Arayan 1, Tran Xuan Ngoc Huy 1, Son Hai Vu 1, Wongi Min 1, Jin Hur 2 and Suk Kim 1*
1Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea, 2Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of KoreaReceived: September 30, 2019; Accepted: December 24, 2019
J. Microbiol. Biotechnol. 2020; 30(4): 482-489
Published April 28, 2020
Copyright © The Korean Society for Microbiology and Biotechnology.
AbstractWe previously identified β-sitosterol (BS) as one of the most abundant compounds found in Korean red ginseng oil. BS is a widely prevalent vegetable-derived phytosterol with many known health benefits. Here, we investigated the efficacy of BS against Brucella (B.) abortus infection. BS showed no effect on bacterial growth but attenuated internalization, intracellular survival and MAPKslinked intracellular signaling in RAW264.7 cells. BS treatment in cells is also associated with increased nitrite concentration during infection at 24 h. Slightly enhanced resistance to B. abortus infection was observed in mice orally given BS, which could be mediated by induced production of proinflammatory cytokines. Taken together, our study demonstrates the contribution of BS treatment against B. abortus infection although further investigation is encouraged to maximize its beneficial effects against intracellular infection.
KeywordsBrucella abortus, &beta,-sitosterol, macrophage, internalization, cytokine
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