2020 ; Vol.30-2: 296~305
|Author||Nan-Yeong Lee, Doo-Ho Choi, Mi-Gyeong Kim, Min-Ji Jeong, Hae-Jun Kwon, Dong-Hyun Kim, Young-Guk Kim, Eric di -Luccio, Manabu Arioka, Hyeok-Jun Yoon, Jong-Guk Kim|
|Place of duty||School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea|
|Title||Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake|
J. Microbiol. Biotechnol.2020 ;
|Abstract||Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora.
Most of studies on T. matsutake have focused on mycelial growth, genes and genomics,
phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its
unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol
and is biosynthesized from the substrate linoleic acid by the sequential reaction of
lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)-
1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study,
cDNA genes correlated with these reactions were cloned from T. matsutake, and expression
studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of
these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)-
1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently
identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with
S. cerevisiae represents a significant step forward.|
|Key_word||(R)-(-)-1-octen-3-ol, Tricholoma matsutake, lipoxygenase, hydroperoxide lyase, Saccharomyces cerevisiae|
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