Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2020 ; Vol.30-2: 296~305

AuthorNan-Yeong Lee, Doo-Ho Choi, Mi-Gyeong Kim, Min-Ji Jeong, Hae-Jun Kwon, Dong-Hyun Kim, Young-Guk Kim, Eric di -Luccio, Manabu Arioka, Hyeok-Jun Yoon, Jong-Guk Kim
Place of dutySchool of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea
TitleBiosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
PublicationInfo J. Microbiol. Biotechnol.2020 ; Vol.30-2
AbstractTricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.
Full-Text
Key_word(R)-(-)-1-octen-3-ol, Tricholoma matsutake, lipoxygenase, hydroperoxide lyase, Saccharomyces cerevisiae
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