2020 ; Vol.30-1: 21~30
|Author||Dae-Hye Choi, Joon-Hee Han, Keun-Hyung Yu, Min Hong, Sun-Yeop Lee, Ka-Hee Park, Soo-Ung Lee, Tae-Hyung Kwon|
|Place of duty||Department of Research and Development, Chuncheon Bio-industry Foundation (CBF), Chuncheon 24232, Republic of Korea|
|Title||Antioxidant and Anti-Obesity Activities of Polygonum cuspidatum Extract through Alleviation of Lipid Accumulation on 3T3-L1 Adipocytes|
J. Microbiol. Biotechnol.2020 ;
|Abstract||Natural products are widely used due to their various biological activities which include antiinflammatory, antioxidant, and anti-obesity effects. In this study, we determined the
antioxidative and anti-obesity effects of Polygonum cuspidatum 50% ethanol extract (PEE). The
antioxidative effect of PEE was evaluated using its radical scavenging activity, total phenolic
content, and reducing power. The anti-obesity effect of PEE was investigated using 3T3-L1
adipocytes. The antioxidative activity of PEE was progressively increased in various
concentrations, mainly due to the presence of phenolic compounds. PEE also alleviated lipid
accumulation on 3T3-L1 adipocytes and downregulated the mRNA and protein production of
adipogenesis-related (SREBP-1c, PPARγ, C/EBPα) and lipogenesis-related (aP2, FAS, ACC)
markers. Furthermore, we found that the inhibitory effect on lipid accumulation via PEE was
caused by the alleviation of NF-κB, p38 MAPK, ERK1/2, and JNK at the protein level. Taken
together, our results imply that PEE is a potential antioxidant that can prevent obesityassociated
|Key_word||3T3L1 cells, lipogenesis, adipogenesis, antioxidant, polygonum cuspidatum|
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