2020 ; Vol.30-1: 93~100
|Author||Ho Sun Sung, Youl Lae Jo|
|Place of duty||Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea|
|Title||Purification and Characterization of an Antibacterial Substance from Aerococcus urinaeequi Strain HS36|
J. Microbiol. Biotechnol.2020 ;
|Abstract||A bacterial strain inhibiting the growth of Vibrio anguillarum, the causative agent of vibriosis,
was isolated from fish intestines. The isolated strain HS36 was identified as Aerococcus
urinaeequi based on the characteristics of the genus according to Bergey’s Manual of Systematic
Bacteriology and by 16S rRNA sequencing. The growth rate and antibacterial activity of strain
HS36 in shaking culture were higher than those in static culture, while the optimal pH and
temperature for antibacterial activity were 7.0 and 30°C, respectively. The active antibacterial
substance was purified from a culture broth of A. urinaeequi HS36 by Sephadex G-75 gel
chromatography, Sephadex G-25 gel chromatography, and reverse-phase high-performance
liquid chromatography. Its molecular weight, as estimated by Tricine SDS-polyacrylamide gel
electrophoresis, was approximately 1,000 Da. The antibacterial substance produced by strain
HS36 was stable after incubation for 1 h at 100°C. Although its antibacterial activity was
optimal at pH 6-8, activity was retained at a pH range from 2 to 11. The purified antibacterial
substance was inactivated by proteinase K, papain, and β-amylase treatment. The newly
purified antibacterial substance, classified as a class II bacteriocin, inhibited the growth of
Klebsiella pneumoniae, Salmonella enterica, and Vibrio alginolyticus.|
|Key_word||Aerococcus urinaeequi, antibacterial substance, aquaculture, bacteriocin, Vibrio (Listonella) anguillarum|
Cordero H, Esteban MA, Cuesta A. 2014. Use of probiotic bacteria against bacterial and viral infections in shellfish and fish aquaculture. Intech. Open Sci. 8: 239-265.
Abarike ED, Jian J, Tang J, Cai J, Yu H, Lihua C, et al. 2018. Influence of traditional Chinese medicine and Bacillus species (TCMBS) on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus. Aquac. Res. 49: 2366-2375.
Baker-Austin C, Oliver JD, Alam M, Ali A, Waldor MK, Qadri F, et al. 2018. Vibrio spp. infections. Nat. Rev. Dis. Primers. 4: 8.
Chatterjee S, Haldar S. 2012. Vibrio related diseases in aquaculture and development of rapid and accurate identification methods. J. Marine Sci. Res. Dev. S: 1.
Amalina NZ, Dzarifah Z, Amal MNA, Yusof MT, ZamriSaad M, Al-saari N, et al. 2019. Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia. Aquaculture Research.
Noga EJ. 2010. Fish Disease: Diagnosis and Treatment, pp. 65-68. 2th Ed. Wiley-Blackwell.
Miranda CD, Tello A, Keen PL. 2013. Mechanisms of antimicrobial resistance in finfish aquaculture environments. Front Microbiol. 4: 233.
Sapkota A, Sapkota AR, Kucharski M, Burke J, McKenzie S, Walker P, et al. 2008. Aquaculture practices and potential human health risks: current knowledge and future priorities. Environ. Int. 34: 1215-1226.
Nwachi OF 2013. An overview of the importance of probiotics in aquaculture. J. Fish Aquat. Sci. 8: 30-32.
Tan L TH., C han K G, L ee LH, G oh B H. 2 016. Streptomyces bacteria as potential probiotics in aquaculture. Front. Microbiol. 7: 79.
Tuan TN, Duc PM, Hatai K. 2013. Overview of the use of probiotics in aquaculture. Inter. J. Res. Fish. Aquac. 3: 89-97.
Cha JH, Yang SY, Woo SH, Song JW, Oh DH, Lee KJ. 2012. Effects of dietary supplementation with Bacillus sp. on growth performance, feed utilization, innate immunity and disease resistance against Streptococcus iniae in olive flounder Paralichthys olivaceus. Kor. J. Fish Aquat. Sci. 45: 35-42.
Todorov SD, de Melo Franco BDG, Tagg JR. 2019. Bacteriocins of Gram-positive bacteria having activity spectra extending beyond closely-related species. Benef. Microbes. 10: 315-328.
Yang SC, Lin CH, Sung CT, Fang JY. 2014. Antibacterial activities of bacteriocins: application in foods and pharmaceuticals. Front. Microbiol. 5: 241.
Hussein AR, Khalaf ZZ, Samir Z, Samir R. 2018. Antibacterial activity of crud Bacteriocin-like substance against food borne bacterial pathogens. Iraqi. J. Sci. 59: 16-24.
De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, et al. 2009. Bergey's Manual of Systematic Bacteriology: Volume 3, pp. 533-536. 2th Ed. The firmicutes.
Crowley S, Mahony J, van Sinderen D. 2013. Current perspectives on antifungal lactic acid bacteria as natural biopreservatives. Trends Food Sci. Technol. 33: 93-109.
Ghanbari MC, Jami M, Domig KJ, Kneifel W. 2013. Seafood biopreservation by lactic acid bacteria - A review. LWTFood Sci. Technol. 54: 315-324.
Perez RH, Zendo T, Sonomoto K. 2014. Novel bacteriocins from lactic acid bacteria (LAB): various structures and applications. Microb. Cell Fact. 13: S3.
Rattanachaikunsopon P, Phumkhachorn P. 2006. Isolation and preliminary characterization of a bacteriocin produced by Lactobacillus plantarum N014 isolated from nham, a traditional Thai fermented pork. J. Food Prot. 69: 1937-1943.
Klaenhammer TR. 1993. Genetics of bacteriocin produced by lactic acid bacteria. FEMS Microbiol. Rev. 12: 39-85.
Ahn JE, Kim JK, Lee HR, Eom HJ, Han NS. 2012. Isolation and characterization of a bacteriocin-producing Lactobacillus sakei B16 from Kimchi. J. Kor. Soc. Food Sci. Nutr. 41: 721-726.
Singh PK, Chittpurna A, Sharma V, Paril, PB, Suresh K. 2012. Identification, purification and characterization of Laterosporulin, a Novel Bacteriocin Produced by Brevibacillus sp. strain Gl-9. PLoS One 7: e31498.
Chen SW, Liu CH, Hu SY. 2018. Dietary administration of probiotic Paenibacillus ehimensis NPUST1 with bacteriocinlike activity improves growth performance and immunity against Aeromonas hydrophila and Streptococcus iniae in Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol. 84: 695-703.
Jimenez-Diaz R, Rios-Sanchez RM, Desmazeaud M, RuizBarba J, Piard JC. 1993. Plantaricin S and T new bacteriocins produced by Lactobacillus plantarum LPCOlO isolated from a green olive fermentation. Appl. Environ. Microbiol. 59: 1416-1424.
Huh CK, Hwang TY. 2016. Identification of antifungal substances of Lactobacillus sakei subsp. ALI033 and antifungal activity against Penicillium brevicompactum strain FI02. Prev. Nutr. Food Sci. 21: 52-56.
Ahn H, Kim J, Kim WJ. 2017. Isolation and characterization of bacteriocin-producing Pediococcus acidilactici HW01 from malt and its potential to control beer spoilage lactic acid bacteria. Food Control. 80: 59-66.
Ryan MP, Rea MC, Hill C, Ross RP. 1996. An application in Cheddar cheese manufacture for a strain of Lactococcus lactis producing a novel broad spectrum bacteriocin, lacticin 3147. Appl. Environ. Microbiol. 62: 612-619.
Hechard Y, Derijard B, Letellier F, Cenatiempo Y. 1992. Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides. J. Gen. Microbiol. 138: 2725-2731.
Belfiore C, Castellano P, Vignolo G. 2007. Reduction of Escherichia coli population following treatment with bacteriocins from lactic acid bacteria and chelators. Food Microbiol. 24: 223-229.