2019 ; Vol.29-12: 1993~2005
|Place of duty||Department Bioengineering, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Potsdam, Germany,Faculty of Agricultural Sciences, Georg-August-Universität Göttingen, Göttingen, Germany|
|Title||Effect of Increasing Amounts of Ammonium Nitrogen Induced by Consecutive Mixture of Poultry Manure and Cattle Slurry on the Microbial Community during Thermophilic Anaerobic Digestion|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Thermophilic anaerobic digestion (TAD) is characterized by higher biogas production rates as
a result of assumedly faster microbial metabolic conversion rates compared to mesophilic AD.
It was hypothesized that the thermophilic microbiome with its lower diversity than the
mesophilic one is more susceptible to disturbances introduced by alterations in the operating
factors, as an example, the supply of nitrogen-rich feedstock such as poultry manure (PM).
Laboratory scaled TAD experiments using cattle slurry and increasing amounts of PM were
carried out to investigate the (in-) stability of the process performance caused by the
accumulation of ammonium and ammonia with special emphasis on the microbial community
structure and its dynamic variation. The results revealed that the moderate PM addition, i.e.,
25% (vol/vol based on volatile substances) PM, resulted in a reorganization of the microbial
community structure which was still working sufficiently. With 50% PM application, the
microbial community was further stepwise re-organized and was able to compensate for the
high cytotoxic ammonia contents only for a short time resulting in consequent process
disturbance and final process failure. This study demonstrated the ability of the acclimated
thermophilic microbial community to tolerate a certain amount of nitrogen-rich substrate.|
|Key_word||Ammonia inhibition, biogas, microbiome, process disturbance|
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