2019 ; Vol.29-12: 1904~1915
|Author||Dong-Hyun Jung, Ga-Young Kim, In-Young Kim, Dong-Ho Seo, Young-Do Nam, Hee Kang, Youngju Song, Cheon-Seok Park|
|Place of duty||Microbial Research Department, Nakdonggang National Institute of Biological Resources, Sangju, 37242, Republic of Korea,Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea|
|Title||Bifidobacterium adolescentis P2P3, a Human Gut Bacterium Having Strong Non-Gelatinized Resistant Starch-Degrading Activity|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Resistant starch (RS) is metabolized by gut microbiota and involved in the production of
short-chain fatty acids, which are related to a variety of physiological and health effects.
Therefore, the availability of RS as a prebiotic is a topic of interest, and research on gut
bacteria that can decompose RS is also important. The objectives in this study were 1) to isolate
a human gut bacterium having strong degradation activity on non-gelatinized RS, 2) to
characterize its RS-degrading characteristics, and 3) to investigate its probiotic effects,
including a growth stimulation effect on other gut bacteria and an immunomodulatory effect.
Bifidobacterium adolescentis P2P3 showing very strong RS granule utilization activity was
isolated. It can attach to RS granules and form them into clusters. It also utilizes high-amylose
corn starch granules up to 63.3%, and efficiently decomposes other various types of
commercial RS without gelatinization. In a coculture experiment, Bacteroides thetaiotaomicron
ATCC 29148, isolated from human feces, was able to grow using carbon sources generated
from RS granules by B. adolescentis P2P3. In addition, B. adolescentis P2P3 demonstrated the
ability to stimulate secretion of Th1 type cytokines from mouse macrophages in vitro that was
not shown in other B. adolescentis. These results suggested that B. adolescentis P2P3 is a useful
probiotic candidate, having immunomodulatory activity as well as the ability to feed other gut
bacteria using RS as a prebiotic.|
|Key_word||Bifidobacterium adolescentis, human intestinal bacteria, immunomodulatory effect, probiotics, resistant starch|
Salyers AA, Leedle JA. 1983. Carbohydrate metabolism in the human colon, pp. 129-146. In Hentges D (ed.), Human intestinal microflora in health and disease, 1st Ed. Elsevier Academic Press, New York.
Fuentes-Zaragoza E, Sánchez-Zapata E, Sendra E, Sayas E, Navarro C, Fernández-López J, et al. 2011. Resistant starch as prebiotic: a review. Starch-Stärke. 63: 406-415.
Bird A, Conlon M, Christophersen C, Topping D. 2010. Resistant starch, large bowel fermentation and a broader perspective of prebiotics and probiotics. Benef. Mirbobes 1:423-431.
Ellis RP, Cochrane MP, Dale MFB, Duffus CM, Lynn A, Morrison IM, et al. 1998. Starch production and industrial use. J. Sci. Food Agric. 77: 289-311.
Singh N, Singh J, Kaur L, Sodhi NS, Gill BS. 2003. Morphological, thermal and rheological properties of starches from different botanical sources. Food Chem. 81: 219-231.
Imberty A, Buléon A, Tran V, Péerez S. 1991. Recent advances in knowledge of starch structure. Starch-Stärke 43: 375-384.
Raigond P, Ezekiel R, Raigond B. 2015. Resistant starch in food: a review. J. Sci. Food Agric. 95: 1968-1978.
Bello-Perez LA, Paredes-López O. 2009. Starches of some food crops, changes during processing and their nutraceutical potential. Food Eng. Rev. 1: 50.
Benmoussa M, Moldenhauer KA, Hamaker BR. 2007. Rice amylopectin fine structure variability affects starch digestion properties. J. Agric. Food Chem. 55: 1475-1479.
Sang Y, Bean S, Seib PA, Pedersen J, Shi Y-C. 2008. Structure and functional properties of sorghum starches differing in amylose content. J. Agric. Food Chem. 56: 6680-6685.
Themeier H, Hollmann J, Neese U, Lindhauer M. 2005. Structural and morphological factors influencing the quantification of resistant starch II in starches of different botanical origin. Carbohydr. Polym. 61: 72-79.
Heitmann T, Wenzig E, Mersmann A. 1997. Characterization of three different potato starches and kinetics of their enzymatic hydrolysis by an α-amylase. Enzyme Microb. Technol. 20: 259-267.
Kong BW, Kim JI, Kim MJ, Kim JC. 2003. Porcine pancreatic α-amylase hydrolysis of native starch granules as a function of granule surface area. Biotechnol. Prog. 19: 1162-1166.
Tester RF, Karkalas J, Qi X. 2004. Starch structure and digestibility enzyme-substrate relationship. Worlds Poult. Sci. J. 60: 186-195.
Božić N, Lončar N, Slavić MŠ, Vujčić Z. 2017. Raw starch degrading α-amylases: an unsolved riddle. Amylase 1: 12-25.
Sun H, Zhao P, Ge X, Xia Y, Hao Z, Liu J, et al. 2010. Recent advances in microbial raw starch degrading enzymes. Appl. Biochem. Biotechnol. 160: 988-1003.
Ze X, Duncan SH, Louis P, Flint HJ. 2012. Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon. ISME J. 6: 1535-1543.
Jung DH, Seo DH, Kim GY, Nam YD, Song EJ, Yoon S, et al. 2018. The effect of resistant starch (RS) on the bovine rumen microflora and isolation of RS-degrading bacteria. Appl. Microbiol. Biotechnol. 102: 4927-4936.
Zhang Z, Schwartz S, Wagner L, Miller W. 2000. A greedy algorithm for aligning DNA sequences. J. Comput. Biol. 7:203-214.
Masuko T, Minami A, Iwasaki N, Majima T, Nishimura S-I, Lee YC. 2005. Carbohydrate analysis by a phenol–sulfuric acid method in microplate format. Anal. Biochem. 339: 69-72.
DuBois M, Gilles KA, Hamilton JK, Rebers Pt, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356.
Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
Martens EC, Koropatkin NM, Smith TJ, Gordon JI. 2009. Complex glycan catabolism by the human gut microbiota:the Bacteroidetes Sus-like paradigm. J. Biol. Chem. 284: 2467324677.
Sun Y, Sun T, Wang F, Zhang J, Li C, Chen X, et al. 2013. A polysaccharide from the fungi of Huaier exhibits anti-tumor potential and immunomodulatory effects. Carbohydr. Polym. 92: 577-582.
Bogdan C. 2001. Nitric oxide and the immune response. Nat. Immunol. 2: 907-916.
Young SL, Simon MA, Baird MA, Tannock GW, Bibiloni R, Spencely K, et al. 2004. Bifidobacterial species differentially affect expression of cell surface markers and cytokines of dendritic cells harvested from cord blood. Clin. Diagn. Lab. Immunol. 11: 686-690.
Rodríguez-Sanoja R, Oviedo N, Sanchez S. 2005. Microbial starch-binding domain. Curr. Opin. Microbiol. 8: 260-267.
Peng H, Zheng Y, Chen M, Wang Y, Xiao Y, Gao Y. 2014. A starch-binding domain identified in α-amylase (AmyP) represents a new family of carbohydrate-binding modules that contribute to enzymatic hydrolysis of soluble starch. FEBS Lett. 588: 1161-1167.
Guillén D, Sánchez S, Rodríguez-Sanoja R. 2010. Carbohydratebinding domains: multiplicity of biological roles. Appl. Microbiol. Biotechnol. 85: 1241-1249.
Jiang S, Wells CD, Roach PJ. 2011. Starch-binding domaincontaining protein 1 (Stbd1) and glycogen metabolism:identification of the Atg8 family interacting motif (AIM) in Stbd1 required for interaction with GABARAPL1. Biochem. Biophys. Res. Commun. 413: 420-425.
D’Argenio V, Salvatore F. 2015. The role of the gut microbiome in the healthy adult status. Clin. Chim. Acta 451:97-102.
Cockburn DW, Orlovsky NI, Foley MH, Kwiatkowski KJ, Bahr CM, Maynard M, et al. 2015. Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol. Microbiol. 95: 209-230.
Ze X, David YB, Laverde-Gomez JA, Dassa B, Sheridan PO, Duncan SH, et al. 2015. Unique organization of extracellular amylases into amylosomes in the resistant starch-utilizing human colonic Firmicutes bacterium Ruminococcus bromii. MBio. 6: e01058-01015.
Shin HS, Eom JE, Shin DU, Yeon SH, Lim SI, Lee SY. 2018. Preventive effects of a probiotic mixture in an ovalbumininduced food allergy model. J. Microbiol. Biotechnol. 28: 65-76.
Sim I, Park KT, Kwon G, Koh JH, Lim YH. 2018. Probiotic potential of Enterococcus faecium isolated from chicken cecum with immunomodulating activity and promoting longevity in Caenorhabditis elegans. J. Microbiol. Biotechnol. 28: 883-892.
Isolauri E, Sütas Y, Kankaanpää P, Arvilommi H, Salminen S. 2001. Probiotics: effects on immunity. Am. J. Clin. Nutr. 73:444s-450s.
Medina M, Izquierdo E, Ennahar S, Sanz Y. 2007. Differential immunomodulatory properties of Bifidobacterium logum strains:relevance to probiotic selection and clinical applications. Clin. Exp. Immunol. 150: 531-538.