2020 ; Vol.30-1: 44~53
|Author||Małgorzata Góral, Urszula Pankiewicz, Monika Sujka, Radosław Kowalski, Dariusz Góral, Katarzyna Kozłowicz|
|Place of duty||Department of Analysis and Food Quality Assessment, Faculty of Food Science and Biotechnology University of Life Sciences Skromna 8; 20-704, Lublin, Poland|
|Title||Influence of Pulsed Electric Field on Accumulation of Calcium in Lactobacillus rhamnosus B 442|
J. Microbiol. Biotechnol.2020 ;
|Abstract||Calcium is an element that performs many important functions in the human body. A study
was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus
rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells
(7.30 mg/g d.m.) was obtained at ion concentration of 200 µg/ml of medium and with the use
of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width
75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass
varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria
ranged from 1010 CFU/ml to 1012 CFU/ml. Microscope photographs prove that calcium ions
were situated within the cells of the bacteria, and electroporation contributed to an increase in
the effectiveness of the ion bioaccumulation process. Samples containing calcium and
subjected to electroporation displayed intensive fluorescence. The significance of this research
was the possibility of using probiotic bacteria enriched with calcium ions for the production of
functional food in subsequent studies.|
|Key_word||PEF, calcium, Lactobacillus rhamnosus|
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