Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2020 ; Vol.30-1: 31~37

AuthorYukihiro Asami, Sun-Ok Kim, Jun-Pil Jang, Sung-Kyun Ko, Bo Yeon Kim, Hiroyuki Osada, Jae-Hyuk Jang, Jong Seog Ahn
Place of dutyAnticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea,Chemical Biology Research Group, RIKEN CSRS, Saitama 351-0198, Japan,Kitasato Institute for Life Sciences, Kitasato University, Tokyo 108-8641, Japan
TitleCRM646-A, a Fungal Metabolite, Induces Nucleus Condensation by Increasing Ca2+ Levels in Rat 3Y1 Fibroblast Cells
PublicationInfo J. Microbiol. Biotechnol.2020 ; Vol.30-1
AbstractWe previously identified a new heparinase inhibitor fungal metabolite, named CRM646-A, which showed inhibition of heparinase and telomerase activities in an in vitro enzyme assay and antimetastatic activity in a cell-based assay. In this study, we elucidated the mechanism by which CRM646-A rapidly induced nucleus condensation, plasma membrane disruption and morphological changes by increasing intracellular Ca2+ levels. Furthermore, PD98059, a mitogen-activated protein kinase (MEK) inhibitor, inhibited CRM646-A-induced nucleus condensation through ERK1/2 activation in rat 3Y1 fibroblast cells. We identified CRM646-A as a Ca2+ ionophore-like agent with a distinctly different chemical structure from that of previously reported Ca2+ ionophores. These results indicate that CRM646-A has the potential to be used as a new and effective antimetastatic drug.
Full-Text
Key_wordNucleus condensation, plasma membrane, Ca2+ signaling, Ca2+ ionophore-like agent, ERK pathway
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