Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-12: 1982~1992

AuthorMinyoung Hong, Wonjae Kim, Woojun Park
Place of dutyDepartment of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea
TitleLow-cost cultivation and sporulation of alkaliphilic Bacillus sp. strain AK13 for self-healing concrete
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-12
AbstractThe alkaliphilic, calcium carbonate precipitating Bacillus sp. strain AK13 can be utilized in concrete. Two types of screening experiment were first conducted to identify substrates that promote the growth of the AK13 strain: the first followed a one-factor-at-a-time factorial design and the second a two-level full factorial design. Based on these screening experiments, barley malt powder and mixed grain powder were identified as the substrates that most effectively promoted the growth of the AK13 strain from a range of 21 agricultural products and by-products. A quadratic statistical model was then constructed using a central composite design and the concentration of the two substrates was optimized. The proposed low-cost medium was approximately 45 times more effective than the commercial medium in terms of the number of cultivatable bacteria per unit price. The spores were then powdered via a spray-drying process to produce a spore powder with a spore count of 2.0 ± 0.7 x 109 CFU/g. The yeast extract and calcium lactate generated the highest CFU/ml for AK13 at a 0.4:0.4 ratio compared to 0.4:0.25 (the original ratio of the B4 medium) and 0.4:0.8. Twenty-eight days after the spores were mixed into the mortar, the number of vegetative cells and spores of the AK13 strain had reached 106 CFU/g within the mortar. Cracks in the mortar under 0.29 mm were healed in 14 days. Calcium carbonate precipitation was observed on the crack surface. The mortar containing the spore powder was thus concluded to be effective in terms of healing micro-cracks.
Full-Text
Key_wordEconomical medium, agricultural products, statistical design of experiments, spray dryer, crack healing material, ; CaCO3¬ precipitation
References
  1. Celik K, Jackson M, Mancio M, Meral C, Emwas AH, Mehta P, et al. 2013. High-volume natural volcanic pozzolan and limestone powder as partial replacements for portland cement in self-compacting and sustainable concrete. Cement. Concrete. Compo. 45: 136-147.
    CrossRef
  2. Meyer C. 2008. The greening of the concrete industry. Cement. Concrete. Comp. 31: 601-605.
    CrossRef
  3. Mignon A, Snoeck D, Dubruel P, Van Vlierberghe S, De Beli N. 2017. Crack mitigation in concrete: superabsorbent polymers as key to success? Materials 10(3): 237.
    Pubmed CrossRef Pubmed Central
  4. Won JP, Kim SH, Lee SJ, Choi S. 2013. Shrinkage and durability characteristics of eco-friendly fireproof highstrength concrete. Constr. Build. Mater. 40: 753-762.
    CrossRef
  5. Hager MD, Greil P, Leyens C, Van Der Zwaag S, Schubert US. 2010. Self-healing materials. Adv. Mater. 22: 5323-5430.
    Pubmed CrossRef
  6. Williams KA, Dreyer DR, Bielawski CW. 2008. The underlying chemistry of self-healing materials. Mrs. Bull. 33: 759-765.
    CrossRef
  7. Palin D, Wiktor V, Jonkers HM. 2017. A bacteria-based selfhealing cementitious composite for application in lowtemperature marine environments. Biomimetics 14:2(3). pii:E13.
    Pubmed CrossRef Pubmed Central
  8. Lee YS, Kim HJ, Park W. 2017. Non-ureolytic calcium carbonate precipitation by Lysinibacillus sp. YS11 isolated from the rhizosphere of Miscanthus sacchariflorus. J. Microbiol. 55: 440-447.
    Pubmed CrossRef
  9. Dick J, De Windt W, De Graef B, Saveyn H, Van der Meeren P, De Belie N, et al. 2006. Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species. Biodegration 17: 357-367.
    Pubmed CrossRef
  10. Jonkers HM, Thijssen A, Muyzer G, Copuroglu O, Schlangen E. 2010. Application of bacteria as self-healing agent for the development of sustainable concrete. Ecol. Eng. 36: 230-235.
    CrossRef
  11. Lee YS, Park W. 2018. Current challenges and future directions for bacterial self-healing concrete. Appl. Microbiol. Biotechnol. 102: 3059-3070.
    Pubmed CrossRef
  12. Chen ZM, Li Q, Liu HM, Yu N, Xie TJ, Yang MY, et al. 2010. Greater enhancement of Bacillus subtilis spore yields in submerged cultures by optimization of medium composition through statistical experimental designs. Appl. Microbiol. Biotechnol. 85: 1353-1360.
    Pubmed CrossRef
  13. Manzoor A, Qazi JI, Haq IU, Mukhtar H, Rasool A. 2017. Significantly enhanced biomass production of a novel biotherapeutic strain Lactobacillus plantarum (AS-14) by developing low cost media cultivation strategy. J. Biol. Eng. 11: 17.
    Pubmed CrossRef Pubmed Central
  14. Coghetto CC, Vasconcelos CB, Brinques GB, Ayub MA. 2016. Lactobacillus plantarum BL011 cultivation in industrial isolated soybean protein acid residue. Braz. J. Microbiol. 47:941-948.
    Pubmed CrossRef Pubmed Central
  15. Lavari L, Páez R, Cuatrin A, Reinheimer J, Vinderola G. 2014. Use of cheese whey for biomass production and spray drying of probiotic lactobacilli. J. Dairy. Res. 81: 267-274.
    Pubmed CrossRef
  16. Khardziani T, Kachlishvili E, Sokhadze K, Elisashvili V, Weeks R, Chikindas ML, et al. 2017. Elucidation of Bacillus subtilis KATMIRA 1933 potential for spore production in submerged fermentation of plant raw materials. Probiotics Antimicrob. Proteins 9: 435-443.
    Pubmed CrossRef
  17. Achal V, Mukherjee A, Basu PC, Reddy MS. 2009. Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii. J. Ind. Microbiol. Biotechnol. 36: 433-438.
    Pubmed CrossRef
  18. Yoosathaporn S, Tiangburanatham P, Bovonsombut S, Chaipanich A, Pathom-Aree W. 2016. A cost effective cultivation medium for biocalcification of Bacillus pasteurii KCTC 3558 and its effect on cement cubes properties. Microbiol. Res. 186-187: 132-138.
    Pubmed CrossRef
  19. Joshi S, Goyal S, Reddy MS. 2018. Corn steep liquor as a nutritional source for biocementation and its impact on concrete structural properties. J. Ind. Microbiol. Biotechnol. 45:657-667.
    Pubmed CrossRef
  20. Wang J, Ersan YC, Boon N, De Belie N. 2016. Application of microorganisms in concrete a promising sustainable strategy to improve concrete durability. Appl. Microbiol. Biotechnol. 100: 2993-3007.
    Pubmed CrossRef
  21. Joshi S, Goyal S, Mukherjee A, Reddy MS. 2017. Microbial healing of cracks in concrete: A review. J. Ind. Microbiol. Biotechnol. 44: 1511-1525.
    Pubmed CrossRef
  22. Lee YS, Park W. 2019. Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13. AMB Express. 9: 49.
    Pubmed CrossRef Pubmed Central
  23. Subra P, Jestin P. 2000. Screening design of experiment (DOE) applied to supercritical antisolvent process. Ind. Eng. Chem. Res. 39: 4178-4184.
    CrossRef
  24. Antony J. 2014. Design of Experiments for Engineers and Scientists, pp. 63-85. 2nd Ed. School of Management and Languages, Heriot-Watt University, Edinburgh, Scotland, UK.
    CrossRef
  25. Sekar A, Kim M, Jeong HC, Kim K. 2018. Strain selection and optimization of mixed culture conditions for Lactobacillus pentosus K1-23 with antibacterial activity and aureobasidium pullulans NRRL 58012 producing immune-enhancing β-glucan. J. Microbiol. Biotechnol. 28: 697-706.
    Pubmed CrossRef
  26. Monteiro SM, Clemente JJ, Henriques AO, Gomes RJ, Carrondo MJ, Cunha AE. 2008. A procedure for high-yield spore production by Bacillus subtilis. Biotechnol Prog. 21:1026-1031.
    Pubmed CrossRef
  27. Ren H, Su YT, Guo XH. 2018. Rapid optimization of spore production from Bacillus amyloliquefaciens in submerged cultures based on dipicolinic acid fluorimetry assay. AMB Express. 8: 21.
    Pubmed CrossRef Pubmed Central
  28. Boquet E, Boronat A, Ramos-Cormenzana A. 1973. Production of calcite (calcium carbonate) crystals by soil bacteria is a general phenomenon. Nature 246: 527-529.
    CrossRef
  29. Horikoshi K. 1996. Alkaliphiles - from an industrial point of view. FEMS Microbiol. Rev. 18: 259-270.
    CrossRef
  30. Chand N, Lonsane BK. 1994. Use of Plackett-Burman design for rapid screening of several nitrogen sources, growth/product promoters, minerals and enzyme inducers for the production of alpha-galactosidase by Aspergillus niger MRSS 234 in solid state fermentation system. Bioprocess Eng. 10:139-144.
    CrossRef
  31. Jo JH, Lee DS, Park D, Choe WS, Park JM. 2007. Optimization of key process variables for enhanced hydrogen production by Enterobacter aerogenes using statistical methods. Bioresour. Technol. 99: 2061-2066.
    Pubmed CrossRef
  32. Almeida AA, Farah A, Silva DA, Nunan EA, Glória MB. 2015. Antibacterial activity of coffee extracts and selected coffee chemical compounds against enterobacteria. J. Agric. Food. Chem. 54: 8738-8743.
    Pubmed CrossRef
  33. Trang H, Pasuwan P. 2018. Screening antimicrobial activity against pathogens from protein hydrolysate of rice bran and Nile Tilapia by-products. Int. Food Res. J. 25: 2157-2163.
  34. Olivieri AC, Magallanes JF. 2012. Uncovering interactions in Plackett–Burman screening designs applied to analytical systems. A Monte Carlo ant colony optimization approach. Talanta 97: 242-248.
    Pubmed CrossRef
  35. Kulahci M, Bisgaard S. 2007. Partial confounding and projective properties of Plackett–Burman designs. Qual. Reliab. Eng. Int. 23: 791-800.
    CrossRef
  36. Lim HS, Cha IT, Roh SW, Shin HH, Seo MJ. 2017. Enhanced production of gamma-aminobutyric acid by optimizing culture conditions of lactobacillus brevis HYE1 isolated from kimchi, a Korean fermented food. J. Microbiol. Biotechnol. 27:450-459.
    Pubmed CrossRef
  37. Shi F, Zhu Y. 2007. Application of statistically-based experimental designs in medium optimization for spore production of Bacillus subtilis from distillery effluent. BioControl. 52: 845-853.
    CrossRef
  38. Kim HK, Park SJ, Han JI, Lee HK. 2012. Microbially mediated calcium carbonate precipitation on normal and lightweight concrete. Constr. Build. Mater. 38: 1073-1082.
    CrossRef
  39. De Belie N, Gruyaert E, Al-Tabbaa A, Actonaci P, Baera C, Bajare D, et al. 2018. A review of self-healing concrete for damage management of structures. Adv. Mater. 5: 17.
    CrossRef
  40. Huang S, Vignolles ML, Chen XD, Le Loir Y, Jan G, Schuck P, et al. 2017. Spray drying of probiotics and other food-grade bacteria: A review. Trends Food Sci. Tech. 63: 1-17.
    CrossRef
  41. Chumthong A, Wiwattanapatapee R, Viernstein H, Pengnoo A, Kanjanamaneesathian M. 2016. Spray-dried powder of Bacillus megaterium for control of rice sheath blight disease:Formulation protocol and efficacy testing in laboratory and greenhouse. Cereal Res. Commun. 44: 131-140.
    CrossRef
  42. Goto S, Roy D. 1981. The effect of w/c ratio and curing temperature on the permeability of hardened cement paste. Cement. Concrete. Res. 11: 575-579.
    CrossRef
  43. Wang J, Jonkers HM, Boon N, De Belie N. 2017. Bacillus sphaericus LMG 22257 is physiologically suitable for selfhealing concrete. Appl. Microbiol. Biotechnol. 101: 5101-5114.
    Pubmed CrossRef
  44. Wang J, Soens H, Verstraete W, De Belie N. 2014. Selfhealing concrete by use of microencapsulated bacterial spores. Cem. Concr. Res. 56: 139-152.
    CrossRef
  45. Achal V, Mukherjee A, Basu PC, Reddy MS. 2009. Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production. J. Ind. Microbiol. Biotechnol. 36:981-988.
    Pubmed CrossRef
  46. Wang J, Snoeck D, Vlierberghe S, Verstraete W, De Belie N. 2014. Application of hydrogel encapsulated carbonate precipitating bacteria for approaching a realistic self-healing in concrete. Constr. Build. Mater. 68: 110-119.
    CrossRef
  47. Wang J, Van Tittelboom K, De Belie N, Verstraete W. 2012. Use of silica gel or polyurethane immobilized bacteria for self-healing concrete. Constr. Build. Mater. 26: 532-540.
    CrossRef
  48. Alazhari M, Sharma T, Heath A, Cooper R, Paine K. 2018. Application of expanded perlite encapsulated bacteria and growth media for self-healing concrete. Constr. Build. Mater. 160: 610-619.
    CrossRef
  49. Wiktor V, Jonkers HM. 2011. Quantification of crack-healing in novel bacteria-based self-healing concrete. Cem. Concr. Compos. 33: 763-770.
    CrossRef



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