2019 ; Vol.29-12: 1916~1924
|Author||Jun-Hyeok Yu, Jeong-A Lim, Hyun-Joo Chang, Jong-Hyun Park|
|Place of duty||epartment of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Republic of Korea|
|Title||Characteristics and Lytic Activity of Phage-Derived Peptidoglycan Hydrolase, LysSAP8, as a Potent Alternative Biocontrol Agent for Staphylococcus aureus|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Outbreaks of staphylococcal food poisoning (SFP) causing serious human diseases and
economic losses have been reported globally. Furthermore, the spread of Staphylococcus aureus
with increased resistance to multiple antimicrobial agents has become a major concern in the
food industries and medicine. Here, we isolated an endolysin LysSAP8, as one of the
peptidoglycan hydrolases, derived from the bacteriophage SAP8 infecting S. aureus. This
endolysin was tagged with a 6×His at the C-terminal of the target protein and purified using
affinity chromatography. LysSAP8 demonstrated lytic activity against a broad spectrum of
bacteria, which included a majority of the staphylococcal strains tested in this study as well as
the methicillin-resistant S. aureus (MRSA); however, no such activity was observed against
other gram-positive or gram-negative bacteria. Additionally, LysSAP8 could maintain
bactericidal activity until 0.1 nM working concentration and after heat treatment at 37°C for
30 min. The ability of LysSAP8 to lyse cells under varying conditions of temperature (4–43°C),
pH (3–9), and NaCl concentrations (0–1,000 mM), and divalent metal ions (Ca2+, Co2+, Cu2+,
Mg2+, Mn2+, Hg2+, and Zn2+) was examined. At the optimized condition, LysSAP8 could disrupt
approximately 3.46 log CFU/ml of the planktonic cells in their exponential phase of growth
within 30 min. In this study, we have suggested that LysSAP8 could be a potent alternative as
a biocontrol agent that can be used to combat MRSA.|
|Key_word||Staphylococcus aureus, bacteriophage, endolysin, MRSA|
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