2019 ; Vol.29-9: 1349~1360
|Author||Mann-Seok Yun, Changhee Kim, Jae-Kwan Hwang|
|Place of duty||Department of Biomaterials Science and Engineering, Yonsei University, Seoul 03722, Republic of Korea,R&D Center, COSMAX NBT, Seoul, 17389, Republic of Korea|
|Title||Agastache rugosa Kuntze Attenuates UVB-Induced Photoaging in Hairless Mice through the Regulation of MAPK/AP-1 and TGF-β/ Smad Pathways|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Chronic exposure to ultraviolet (UV) radiation, regarded as a major cause of extrinsic aging or
photoaging characterized by wrinkle formation and skin dehydration, exerts adverse effects
on skin by causing the overproduction of reactive oxygen species. Agastache rugosa Kuntze,
known as Korean mint, possesses a wide spectrum of biological properties including antioxidation,
anti-inflammation, and anti-atherosclerosis. Previous studies have reported that A.
rugosa protected human keratinocytes against UVB irradiation by restoring the anti-oxidant
defense system. However, the anti-photoaging effect of A. rugosa extract (ARE) in animal
models has not yet been evaluated. ARE was orally administered to hairless mice at doses of
100 or 250 mg/kg/day along with UVB exposure for 12 weeks. ARE histologically improved
UVB-induced wrinkle formation, epidermal thickening, erythema, and hyperpigmentation. In
addition, ARE recovered skin moisture by improving skin hydration and transepidermal
water loss (TEWL). Along with this, ARE increased hyaluronic acid levels by upregulating HA
synthase genes. ARE markedly increased the density of collagen and the amounts of
hydroxypoline via two pathways. First, ARE significantly downregulated the mRNA
expression of matrix metalloproteinases responsible for collagen degradation by inactivating
the mitogen-activated protein kinase/activator protein 1 pathway. Second, ARE stimulated
the transforming growth factor beta/Smad signaling, consequently raising the mRNA levels of
collagen-related genes. In addition, ARE not only increased the mRNA expression of antioxidant
enzymes but also decreased inflammatory cytokines by blocking the protein
expression of nuclear factor kappa B. Collectively, our findings suggest that A. rugosa may be a
potential preventive and therapeutic agent for photoaging.|
|Key_word||Agastache rugosa Kuntze, collagen, Korean mint, photoaging, skin moisture|
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