2019 ; Vol.29-11: 1830~1840
|Author||Yu Rim Lee, Seunghee Bae, Ji Yea Kim, Junwoo Lee, Dae-Hyun Cho, Hee-Sik Kim, In-Sook An, Sungkwan An|
|Place of duty||Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea|
|Title||Monoterpenoid Loliolide Regulates Hair Follicle Inductivity of Human Dermal Papilla Cells by Activating the Akt/β-Catenin Signaling Pathway|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Loliolide is one of the most ubiquitous monoterpenoid compounds found in algae, and its
potential therapeutic effect on various dermatological conditions via agent-induced biological
functions, including anti-oxidative and anti-apoptotic properties, was demonstrated. Here, we
investigated the effects of loliolide on hair growth in dermal papilla (DP) cells, the main
components regulating hair growth and loss conditions. For this purpose, we used a threedimensional
(3D) DP spheroid model that mimics the in vivo hair follicle system. Biochemical
assays showed that low doses of loliolide increased the viability and size of 3D DP spheroids
in a dose-dependent manner. This result correlated with increases in expression levels of hair
growth-related autocrine factors including VEGF, IGF-1, and KGF. Immunoblotting and
luciferase-reporter assays further revealed that loliolide induced AKT phosphorylation, and
this effect led to stabilization of β-catenin, which plays a crucial role in the hair-inductive
properties of DP cells. Further experiments showed that loliolide increased the expression
levels of the DP signature genes, ALP, BMP2, VCAN, and HEY1. Furthermore, conditioned
media from loliolide-treated DP spheroids significantly enhanced proliferation and the
expression of hair growth regulatory genes in keratinocytes. These results suggested that
loliolide could function in the hair growth inductivity of DP cells via the AKT/ β-catenin
|Key_word||Loliolide, hair follicle induction, dermal papilla, spheroids, AKT, migration|
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