2020 ; Vol.30-2: 172~177
|Author||Minjee Kim, Dinh-Van Nguyen, Yoonki Heo, Ki Hoon Park, Hyun-Dong Paik, Young Bong Kim|
|Place of duty||Department of Biomedical Science and Engineering, Konkuk University, Seoul 05029, Republic of Korea|
|Title||Antiviral Activity of Fritillaria thunbergii Extract against Human Influenza Virus H1N1 (PR8) In Vitro, In Ovo and In Vivo|
J. Microbiol. Biotechnol.2020 ;
|Abstract||Influenza viruses cause respiratory diseases in humans and animals with high morbidity and
mortality rates. Conventional anti-influenza drugs are reported to exert side effects and newly
emerging viral strains tend to develop resistance to these commonly used agents. Fritillaria
thunbergii (FT) is traditionally used as an expectorant for controlling airway inflammatory
disorders. Here, we evaluated the therapeutic effects of FT extracts against influenza virus
type A (H1N1) infection in vitro, in ovo, and in vivo. In the post-treatment assay, FT extracts
showed high CC50 (7,500 μg/ml), indicating low toxicity, and exerted moderate antiviral
effects compared to oseltamivir (SI 50.6 vs. 222) in vitro. Antiviral activity tests in ovo revealed
strong inhibitory effects of both FT extract and oseltamivir against H1N1 replication in
embryonated eggs. Notably, at a treatment concentration of 150 mg/kg, only half the group
administered oseltamivir survived whereas the FT group showed 100% survival, clearly
demonstrating the low toxicity of FT extracts. Consistent with these findings, FT-administered
mice showed a higher survival rate with lower body weight reduction relative to the
oseltamivir group upon treatment 24 h after viral infection. Our collective results suggest that
FT extracts exert antiviral effects against influenza H1N1 virus without inducing toxicity in
vitro, in ovo or in vivo, thereby supporting the potential utility of FT extract as a novel
candidate therapeutic drug or supplement against influenza.|
|Key_word||Antiviral activity, Influenza A (H1N1), Fritillaria thunbergii, oseltamivir|
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