2020 ; Vol.30-2: 163~171
|Author||Hyoung-Geun Kim, Davin Jang, Young Sung Jung, Hyun-Ji Oh, Seon Min Oh, Yeong-Geun Lee, Se Chan Kang, Dae-Ok Kim, Dae Young Lee, Nam-In Baek|
|Place of duty||Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea|
|Title||Anti-Inflammatory Effect of Flavonoids from Brugmansia arborea L. Flowers|
J. Microbiol. Biotechnol.2020 ;
|Abstract||Brugmansia arborea L. (Solanaceae), commonly known as “angel’s trumpet,” is widely grown in
North America, Africa, Australia, and Asia. It has been mainly used for ornamental purposes
as well as analgesic, anti-rheumatic, vulnerary, decongestant, and anti-spasmodic materials.
B. arborea is also reported to show anti-cholinergic activity, for which many alkaloids were
reported to be principally responsible. However, to the best of our knowledge, a
phytochemical study of B. arborea flowers has not yet been performed. Four flavonol
glycosides (1–4) and one dihydroflavanol (5) were for the first time isolated from B. arborea
flowers in this study. The flavonoids showed significant antioxidant capacities, suppressed
nitric oxide production in lipopolysaccharide (LPS)-treated RAW 264.7 cells, and reduced
inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) protein production
increased by LPS treatment. The contents of compounds 1–4 in n-BuOH fraction were
determined to be 3.8 ± 0.9%, 2.2 ± 0.5%, 20.3 ± 1.1%, and 2.3 ± 0.4%, respectively, and that of
compound 5 in EtOAc fraction was determined to be 12.7 ± 0.7%, by HPLC experiment. These
results suggest that flavonol glycosides (1–4) and dihydroflavanol (5) can serve as index
components of B. arborea flowers in standardizing anti-inflammatory materials.|
|Key_word||COX-2, flavonol glycoside, HPLC, nitric oxide, RAW 264.7, iNOS|
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