2019 ; Vol.29-10: 1636~1643
|Author||Song-Yi Kim, Han Kim, Bong-Gyu Kim, Joong-Hoon Ahn|
|Place of duty||Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 05029, Republic of Korea|
|Title||Biosynthesis of Two Hydroxybenzoic Acid-Amine Conjugates in Engineered Escherichia coli|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Two hydroxybenzoyl amines, 4-hydroxybenzoyl tyramine (4-HBT) and N-2-hydroxybenzoyl
tryptamine (2-HBT), were synthesized using Escherichia coli. While 4-HBT was reported to
demonstrate anti-atherosclerotic activity, 2-HBT showed anticonvulsant and antinociceptive
activities. We introduced genes chorismate pyruvate-lyase (ubiC), tyrosine decarboxylase
(TyDC), isochorismate synthase (entC), isochorismate pyruvate lyase (pchB), and tryptophan
decarboxylase (TDC) for each substrate, 4-hydroxybenzoic acid (4-HBA), tyramine,
2-hydroxybenzoic acid (2-HBA), and tryptamine, respectively, in E. coli. Genes for CoA ligase
(hbad) and amide formation (CaSHT and OsHCT) were also introduced to form hydroxybenzoic
acid and amine conjugates. In addition, we engineered E. coli to provide increased substrates.
These approaches led to the yield of 259.3 mg/l 4-HBT and 227.2 mg/l 2-HBT and could be
applied to synthesize diverse bioactive hydroxybenzoyl amine conjugates.|
|Key_word||Hydroxybenzoic acid, N-2-hydroxybenzoyl tryptamine, 4-hydroxybenzoyl tyramine, metabolic engineering|
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