Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2020 ; Vol.30-1: 85~92

AuthorHyun-Woo Chun, Jintak Lee, Pham Thu Huyen, Jiyon Lee, Jae-Hwan Yoon, Jin Lee, Deok-Kun Oh, Jaewook Oh, Do-Young Yoon
Place of dutyDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea
TitleResolvin D5, a Lipid Mediator, Inhibits Production of Interleukin-6 and CCL5 Via the ERK-NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated THP-1 Cells
PublicationInfo J. Microbiol. Biotechnol.2020 ; Vol.30-1
AbstractOne of the omega-3 essential fatty acids, docosahexaenoic acid (DHA), is a significant constituent of the cell membrane and the precursor of several potent lipid mediators. These mediators are considered to be important in preventing or treating several diseases. Resolvin D5, an oxidized lipid mediator derived from DHA, has been known to exert anti-inflammatory effects. However, the detailed mechanism underlying these effects has not yet been elucidated in human monocytic THP-1 cells. In the present study, we investigated the effects of resolvin D5 on inflammation-related signaling pathways, including the extracellular signal-regulated kinase (ERK)-nuclear factor (NF)-κB signaling pathway. Resolvin D5 downregulated the production of interleukin (IL)-6 and chemokine (C-C motif) ligand 5 (CCL5). Additionally, these inhibitory effects were found to be modulated by mitogen-activated protein kinase (MAPK) and NF-κB in lipopolysaccharide (LPS)-treated THP-1 cells. Resolvin D5 inhibited the LPS-stimulated phosphorylation of ERK and translocation of p65 and p50 into the nucleus, resulting in the inhibition of IL-6 and CCL5 production. These results revealed that resolvin D5 exerts anti-inflammatory effects in LPS-treated THP-1 cells by regulating the phosphorylation of ERK and nuclear translocation of NF-κB.
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Supplemental Data
Key_wordResolvin D5, anti-inflammatory, THP-1 cells, ERK, NF-κB
References
  1. Duvall MG, Levy BD. 2016. DHA- and EPA-derived resolvins, protectins, and maresins in airway inflammation. Eur. J. Pharmacol. 785: 144-155.
    Pubmed CrossRef Pubmed Central
  2. Simopoulos AP. 2002. Omega-3 fatty acids in inflammation and autoimmune diseases. J. Am. Coll. Nutr. 21: 495-505.
    Pubmed CrossRef
  3. Simopoulos AP. 2008. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp. Biol. Med (Maywood). 233: 674-688.
    Pubmed CrossRef
  4. Gobbetti T, Dalli J, Colas RA, Federici Canova D, Aursnes M, Bonnet D, et al. 2017. Protectin D1n-3 DPA and resolvin D5n-3 DPA are effectors of intestinal protection. Proc. Natl. Acad. Sci. USA 114: 3963-3968.
    Pubmed CrossRef Pubmed Central
  5. Luo X, Gu Y, Tao X, Serhan CN, Ji RR. 2019. Resolvin D5 Inhibits neuropathic and Inflammatory pain in male but not female mice: Distinct actions of D-series resolvins in chemotherapy-Induced peripheral neuropathy. Front. Pharmacol. 10: 745.
    Pubmed CrossRef Pubmed Central
  6. Serhan CN. 2014. Pro-resolving lipid mediators are leads for resolution physiology. Nature 510: 92-101.
    Pubmed CrossRef Pubmed Central
  7. Ogawa N, Sugiyama T, Morita M, Suganuma Y, Kobayashi Y. 2017. Total synthesis of resolvin D5. J. Org. Chem. 82: 2032-2039.
    Pubmed CrossRef
  8. Tang S, Shen XY, Huang HQ, Xu SW, Yu Y, Zhou CH, et al. 2011. Cryptotanshinone suppressed inflammatory cytokines secretion in RAW264.7 macrophages through inhibition of the NF-kappaB and MAPK signaling pathways. Inflammation 34: 111-118.
    Pubmed CrossRef
  9. Chanput W, Mes JJ, Wichers HJ. 2014. THP-1 cell line: an in vitro cell model for immune modulation approach. Int. Immunopharmacol. 23: 37-45.
    Pubmed CrossRef
  10. Kueanjinda P, Roytrakul S, Palaga T. 2015. A Novel role of numb as a regulator of pro-inflammatory cytokine production in macrophages in response to Toll-like receptor 4. Sci. Rep. 5: 12784.
    Pubmed CrossRef Pubmed Central
  11. Yang L, Guo H, Li Y, Meng X, Yan L, Dan Z, et al. 2016. Oleoylethanolamide exerts anti-inflammatory effects on LPS-induced THP-1 cells by enhancing PPARalpha signaling and inhibiting the NF-kappaB and ERK1/2/AP-1/STAT3 pathways. Sci. Rep. 6: 34611.
    Pubmed CrossRef Pubmed Central
  12. Yoon YK, Woo HJ, Kim Y. 2015. Orostachys japonicus inhibits expression of the TLR4, NOD2, iNOS, and COX-2 genes in LPS-stimulated human PMA-differentiated THP-1 cells by inhibiting NF-kappaB and MAPK activation. Evid. Based Complement Alternat. Med. 2015: 682019.
    Pubmed CrossRef Pubmed Central
  13. Karima R, Matsumoto S, Higashi H, Matsushima K. 1999. The molecular pathogenesis of endotoxic shock and organ failure. Mol. Med. Today. 5: 123-132.
    CrossRef
  14. Kaminska B, Gozdz A, Zawadzka M, Ellert-Miklaszewska A, Lipko M. 2009. MAPK signal transduction underlying brain inflammation and gliosis as therapeutic target. Anat. Rec. (Hoboken). 292: 1902-1913.
    Pubmed CrossRef
  15. Huang P, Han J, Hui L. 2010. MAPK signaling in inflammation-associated cancer development. Protein Cell 1: 218-226.
    Pubmed CrossRef Pubmed Central
  16. Hu B, Zhang H, Meng X, Wang F, Wang P. 2014. Aloeemodin from rhubarb (Rheum rhabarbarum) inhibits lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages. J. Ethnopharmacol. 153: 846-853.
    Pubmed CrossRef
  17. Chun HW, Kim SJ, Pham TH, B ak Y , Oh J , R yu HW, et al. 2019. Epimagnolin A inhibits IL-6 production by inhibiting p38/NF-kappaB and AP-1 signaling pathways in PMAstimulated THP-1 cells. Environ. Toxicol. 34: 796-803.
    Pubmed CrossRef
  18. Li W, Tan D, Zenali MJ, Brown RE. 2009. Constitutive activation of nuclear factor-kappa B (NF-kB) signaling pathway in fibrolamellar hepatocellular carcinoma. Int. J. Clin. Exp. Pathol. 3: 238-243.
  19. White B, Schmidt M, Murphy C, Livingstone W, O’Toole D, Lawler M, et al. 2000. Activated protein C inhibits lipopolysaccharide-induced nuclear translocation of nuclear factor kappaB (NF-kappaB) and tumour necrosis factor alpha (TNF-alpha) production in the THP-1 monocytic cell line. Br. J. Haematol. 110: 130-134.
    Pubmed CrossRef
  20. Pham TH, Kim MS, Le MQ, Song YS, Bak Y, Ryu HW, et al. 2017. Fargesin exerts anti-inflammatory effects in THP-1 monocytes by suppressing PKC-dependent AP-1 and NF-kB signaling. Phytomedicine 24: 96-103.
    Pubmed CrossRef
  21. An JU, Song YS, Kim KR, Ko YJ, Yoon DY, Oh DK. 2018. Biotransformation of polyunsaturated fatty acids to bioactive hepoxilins and trioxilins by microbial enzymes. Nat. Commun. 9: 128.
    Pubmed CrossRef Pubmed Central
  22. Yoon DY, Cho MC, Kim JH, Kim EJ, Kang JW, Seo EH, et al. 2008. Effects of a tetramethoxyhydroxyflavone p7f on the expression of inflammatory mediators in LPS-treated human synovial fibroblast and macrophage cells. J. Microbiol. Biotechnol. 18: 686-694.
  23. Kang JW, Choi SC, Cho MC, Kim HJ, Kim JH, Lim JS, et al. 2009. A proinflammatory cytokine interleukin-32beta promotes the production of an anti-inflammatory cytokine interleukin-10. Immunology 128: e532-540.
    Pubmed CrossRef Pubmed Central
  24. Neurath MF, Finotto S. 2011. IL-6 signaling in autoimmunity, chronic inflammation and inflammation-associated cancer. Cytokine Growth Factor Rev. 22: 83-89.
    Pubmed CrossRef
  25. Lee DH, Park MH, Hwang CJ, Hwang JY, Yoon HS, Yoon DY, et al. 2016. CCR5 deficiency increased susceptibility to lipopolysaccharide-induced acute renal injury. Arch. Toxicol. 90: 1151-1162.
    Pubmed CrossRef
  26. Heinrich PC, Castell JV, Andus T. 1990. Interleukin-6 and the acute phase response. Biochem. J. 265: 621-636.
    Pubmed CrossRef Pubmed Central
  27. Kishimoto T, Akira S, Taga T. 1992. Interleukin-6 and its receptor: a paradigm for cytokines. Science 258: 593-597.
    Pubmed CrossRef
  28. Gabay C. 2006. Interleukin-6 and chronic inflammation. Arthritis Res. Ther. 8 Suppl 2: S3.
    Pubmed CrossRef Pubmed Central
  29. Aldinucci D, Colombatti A. 2014. The inflammatory chemokine CCL5 and cancer progression. Mediators Inflamm. 2014: 292376.
    Pubmed CrossRef Pubmed Central
  30. Soria G, Ben-Baruch A. 2008. The inflammatory chemokines CCL2 and CCL5 in breast cancer. Cancer Lett. 267: 271-285.
    Pubmed CrossRef
  31. Wu SH, Chen XQ, Liu B, Wu HJ, Dong L. 2013. Efficacy and safety of 15(R/S)-methyl-lipoxin A(4) in topical treatment of infantile eczema. Br. J. Dermatol. 168: 172-178.
    Pubmed CrossRef
  32. Chiang N, Fredman G, Backhed F, Oh SF, Vickery T, Schmidt BA, et al. 2012. Infection regulates pro-resolving mediators that lower antibiotic requirements. Nature 484: 524-528.
    Pubmed CrossRef Pubmed Central
  33. Werz O, Gerstmeier J, Libreros S, De la Rosa X, Werner M, Norris PC, et al. 2018. Human macrophages differentially produce specific resolvin or leukotriene signals that depend on bacterial pathogenicity. Nat. Commun. 9: 59.
    Pubmed CrossRef Pubmed Central
  34. Thalhamer T, McGrath MA, Harnett MM. 2008. MAPKs and their relevance to arthritis and inflammation. Rheumatology (Oxford) 47: 409-414.
    Pubmed CrossRef
  35. Baker RG, Hayden MS, Ghosh S. 2011. NF-kappaB, inflammation, and metabolic disease. Cell Metab. 13: 11-22.
    Pubmed CrossRef Pubmed Central
  36. Hanada T, Yoshimura A. 2002. Regulation of cytokine signaling and inflammation. Cytokine Growth Factor Rev. 13: 413-421.
    CrossRef
  37. Lo CJ, Chiu KC, Fu M, Lo R, Helton S. 1999. Fish oil decreases macrophage tumor necrosis factor gene transcription by altering the NF kappa B activity. J. Surg. Res. 82: 216-221.
    Pubmed CrossRef
  38. Babcock TA, Novak T, Ong E, Jho DH, Helton WS, Espat NJ. 2002. Modulation of lipopolysaccharide-stimulated macrophage tumor necrosis factor-alpha production by omega-3 fatty acid is associated with differential cyclooxygenase-2 protein expression and is independent of interleukin-10. J. Surg. Res. 107: 135-139.
    Pubmed CrossRef
  39. Lee JY, Sohn KH, Rhee SH, Hwang D. 2001. Saturated fatty acids, but not unsaturated fatty acids, induce the expression of cyclooxygenase-2 mediated through Toll-like receptor 4. J. Biol. Chem. 276: 16683-16689.
    Pubmed CrossRef
  40. Calder PC. 2012. Long-chain fatty acids and inflammation. Proc. Nutr. Soc. 71: 284-289.
    Pubmed CrossRef



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