2020 ; Vol.30-1: 85~92
|Author||Hyun-Woo Chun, Jintak Lee, Pham Thu Huyen, Jiyon Lee, Jae-Hwan Yoon, Jin Lee, Deok-Kun Oh, Jaewook Oh, Do-Young Yoon|
|Place of duty||Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea |
|Title||Resolvin D5, a Lipid Mediator, Inhibits Production of Interleukin-6 and CCL5 Via the ERK-NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated THP-1 Cells|
J. Microbiol. Biotechnol.2020 ;
|Abstract||One of the omega-3 essential fatty acids, docosahexaenoic acid (DHA), is a significant
constituent of the cell membrane and the precursor of several potent lipid mediators. These
mediators are considered to be important in preventing or treating several diseases. Resolvin
D5, an oxidized lipid mediator derived from DHA, has been known to exert anti-inflammatory
effects. However, the detailed mechanism underlying these effects has not yet been elucidated
in human monocytic THP-1 cells. In the present study, we investigated the effects of resolvin
D5 on inflammation-related signaling pathways, including the extracellular signal-regulated
kinase (ERK)-nuclear factor (NF)-κB signaling pathway. Resolvin D5 downregulated the
production of interleukin (IL)-6 and chemokine (C-C motif) ligand 5 (CCL5). Additionally,
these inhibitory effects were found to be modulated by mitogen-activated protein kinase
(MAPK) and NF-κB in lipopolysaccharide (LPS)-treated THP-1 cells. Resolvin D5 inhibited the
LPS-stimulated phosphorylation of ERK and translocation of p65 and p50 into the nucleus,
resulting in the inhibition of IL-6 and CCL5 production. These results revealed that resolvin
D5 exerts anti-inflammatory effects in LPS-treated THP-1 cells by regulating the
phosphorylation of ERK and nuclear translocation of NF-κB.|
|Key_word||Resolvin D5, anti-inflammatory, THP-1 cells, ERK, NF-κB|
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