Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2020 ; Vol.30-1: 127~135

AuthorBingbing Hou, Xichen Yang, Hui Xia, Haizhen Wu, Jiang Ye, Huizhan Zhang
Place of dutyState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, P.R.China,Department of Applied Biology, East China University of Science and Technology, Shanghai, P.R.China
TitlesRNA EsrE Is Transcriptionally Regulated by the Ferric Uptake Regulator Fur in Escherichia coli
PublicationInfo J. Microbiol. Biotechnol.2020 ; Vol.30-1
AbstractSmall RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis-regulatory elements exist in the promoter region of esrE. We then screened and analyzed seven potential corresponding trans-regulatory elements by using pull-down assays based on DNA affinity chromatography. Among these candidate regulators, we investigated the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and β-galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe2+.
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Key_wordSmall RNA, EsrE, DNA affinity chromatography, Fur, transcriptional regulation
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