Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-12: 2006~2013

AuthorHan Saem Lee, Hye-Min Woo, Kisoon Kim, Sehee Park, Man-Seong Park, Sung Soon Kim, You-Jin Kim
Place of dutyCenter for Infectious Diseases Research, National Institute of Health, Korea Centers for Diseases Control and Prevention, Cheongju 28159, Republic of Korea
TitleImproving Pneumovirus Isolation Using a Centrifugation and AZD1480 Combined Method
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-12
AbstractThe isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.
Full-Text
Key_wordVirus isolation, centrifugation, AZD1480, human respiratory syncytial virus, human metapneumovirus
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