2019 ; Vol.29-12: 2006~2013
|Author||Han Saem Lee, Hye-Min Woo, Kisoon Kim, Sehee Park, Man-Seong Park, Sung Soon Kim, You-Jin Kim|
|Place of duty||Center for Infectious Diseases Research, National Institute of Health, Korea Centers for Diseases Control and Prevention, Cheongju 28159, Republic of Korea |
|Title||Improving Pneumovirus Isolation Using a Centrifugation and AZD1480 Combined Method|
J. Microbiol. Biotechnol.2019 ;
|Abstract||The isolation of respiratory viruses, especially from clinical specimens, often shows poor
efficiency with classical cell culture methods. The lack of suitable methods to generate virus
particles inhibits the development of diagnostic assays, treatments, and vaccines. We
compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor
AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory
syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined
method using AZD1480 treatment and CEI was used on throat swabs to verify that this
method could increase virus isolation efficiency from human clinical specimens. Both CEI and
AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined
method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The
combined method is particularly suited for the isolation of interferon-sensitive or slowly
growing viruses from human clinical specimens.|
|Key_word||Virus isolation, centrifugation, AZD1480, human respiratory syncytial virus, human metapneumovirus|
Morens DM, Fauci AS. 2013. Emerging infectious diseases:threats to human health and global stability. PLoS Pathog. 9:e1003467.
Morse SS. 1995. Factors in the emergence of infectious diseases. Emerg. Infect. Dis. 1: 7-15.
Smith KF, Goldberg M, Rosenthal S, Carlson L, Chen J, Chen C, et al. 2014. Global rise in human infectious disease outbreaks. J. R. Soc. Interface 11: 20140950.
Fonkwo PN. 2008. Pricing infectious disease. The economic and health implications of infectious diseases. EMBO Rep. 9 Suppl 1: S13-17.
Christaki E. 2015. New technologies in predicting, preventing and controlling emerging infectious diseases. Virulence 6:558-565.
Leland DS, Ginocchio CC. 2007. Role of cell culture for virus detection in the age of technology. Clin. Microbiol. Rev. 20: 49-78.
Miller RR, Montoya V, Gardy JL, Patrick DM, Tang P. 2013. Metagenomics for pathogen detection in public health. Genome Med. 5: 81.
Tahir RA, Goyal SM. 1995. Rapid detection of p seudorabies virus by the shell vial technique. J. Vet. Diagn. Invest. 7: 173-176.
Dunn JJ, Woolstenhulme RD, Langer J, Carroll KC. 2004. Sensitivity of respiratory virus culture when screening with R-mix fresh cells. J. Clin. Microbiol. 42: 79-82.
Stewart CE, Randall RE, Adamson CS. 2014. Inhibitors of the interferon response enhance virus replication in vitro. PLoS One 9: e112014.
Thomas BJ, Porritt RA, Hertzog PJ, Bardin PG, Tate MD. 2014. Glucocorticosteroids enhance replication of respiratory viruses: effect of adjuvant interferon. Sci. Rep. 4: 7176.
Farsani SM, Deijs M, Dijkman R, Molenkamp R, Jeeninga RE, Ieven M, et al. 2015. Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses. Influenza Other Respir. Viruses 9: 51-57.
Pyrc K, Sims AC, Dijkman R, Jebbink M, Long C, Deming D, et al. 2010. Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures. J. Virol. 84: 11255-11263.
Byington CL, Wilkes J, Korgenski K, Sheng X. 2015. Respiratory syncytial virus-associated mortality in hospitalized infants and young children. Pediatrics 135: e24-31.
Nair H, Nokes DJ, Gessner BD, Dherani M, Madhi SA, Singleton RJ, et al. 2010. Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis. Lancet 375: 1545-1555.
Derenzini E, Lemoine M, Buglio D, Katayama H, Ji Y, Davis RE, et al. 2011. The JAK inhibitor AZD1480 regulates proliferation and immunity in Hodgkin lymphoma. Blood Cancer J. 1: e46.
Randall RE, Goodbourn S. 2008. Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J. Gen. Virol. 89: 1-47.
Moore ML, Chi MH, Luongo C, Lukacs NW, Polosukhin VV, Huckabee MM, et al. 2009. A chimeric A2 strain of respiratory syncytial virus (RSV) with the fusion protein of RSV strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction. J. Virol. 83: 4185-4194.
Shirogane Y, Takeda M, Iwasaki M, Ishiguro N, Takeuchi H, Nakatsu Y, et al. 2008. Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2. J. Virol. 82: 8942-8946.
Kim JM, Jung HD, Cheong HM, Lee A, Lee NJ, Chu H, et al. 2018. Nation-wide surveillance of human acute respiratory virus infections between 2013 and 2015 in Korea. J. Med. Virol. 90: 1177-1183.
Landry ML, Ferguson D, Cohen S, Peret TC, Erdman DD. 2005. Detection of human metapneumovirus in clinical samples by immunofluorescence staining of shell vial centrifugation cultures prepared from three different cell lines. J. Clin. Microbiol. 43: 1950-1952.
Sato K, Watanabe O, Ohmiya S, Chiba F, Suzuki A, Okamoto M, et al. 2017. Efficient isolation of human metapneumovirus using MNT-1, a h uman m alignant m elanoma c ell line w ith early and distinct cytopathic effects. Microbiol. Immunol. 61: 497-506.
Ye L, Wang X, Wang S, Luo G , Wang Y, Liang H , et al. 2008. Centrifugal enhancement of hepatitis C virus infection of human hepatocytes. J. Virol. Methods. 148: 161-165.
Kotani H, Newton P B, 3rd, Zhang S , Chiang YL, Otto E, Weaver L, et al. 1994. Improved methods of retroviral vector transduction and production for gene therapy. Hum. Gene Ther. 5: 19-28.
Reading SA, Edwards MJ, Dimmock NJ. 2001. Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement. J. Virol. Methods 98: 167-169.
Sundin DR, Mecham JO. 1989. Enhanced infectivity of bluetongue virus in cell culture by centrifugation. J. Clin. Microbiol. 27: 1659-1660.
Hodgkin PD, Scalzo AA, Swaminathan N, Price P, Shellam GR. 1988. Murine cytomegalovirus binds reversibly to mouse embryo fibroblasts: implications for quantitation and explanation of centrifugal enhancement. J. Virol. Methods 22:215-230.
Gritsina G, Xiao F, O'Brien SW, Gabbasov R, Maglaty MA, Xu RH, et al. 2015. Targeted Blockade of JAK/STAT3 Signaling Inhibits Ovarian Carcinoma Growth. Mol. Cancer Ther. 14: 1035-1047.
Hedvat M, Huszar D, Herrmann A, Gozgit JM, Schroeder A, Sheehy A, et al. 2009. The JAK2 inhibitor AZD1480 potently blocks Stat3 signaling and oncogenesis in solid tumors. Cancer Cell 16: 487-497.
Huck B, Neumann-Haefelin D, Schmitt-Graeff A, Weckmann M, Mattes J, Ehl S , et al. 2007. Human metapneumovirus induces more severe disease and stronger innate immune response in BALB/c mice as compared with respiratory syncytial virus. Respir. Res. 8: 6.
Kuo TM, Hu CP, Chen YL, Hong MH, Jeng KS, Liang CC, et al. 2009. HBV replication is significantly reduced by IL-6. J. Biomed. Sci. 16: 41.
Desmyter J, Melnick JL, Rawls WE. 1968. Defectiveness of interferon production and of rubella virus interference in a line of African green monkey kidney cells (Vero). J. Virol. 2: 955-961.
Plimack ER, Lorusso PM, McCoon P, Tang W, Krebs AD, Curt G, et al. 2013. AZD1480: a phase I study of a novel JAK2 inhibitor in solid tumors. Oncologist 18: 819-820.