2019 ; Vol.29-12: 1947~1956
|Author||Kondreddy Eswar Reddy, Hye-Ran Kim, Jin Young Jeong, Kyoung-Min So, Seul Lee, Sang Yun Ji, Minji Kim, Hyun-Jung Lee, Sungdae Lee, Ki-Hyun Kim, Minseok Kim|
|Place of duty||Animal Nutrition and Physiology Team, National Institute of Animal Science, Wanju 55365, Republic of Korea|
|Title||Impact of Breed on the Fecal Microbiome of Dogs under the Same Dietary Condition|
J. Microbiol. Biotechnol.2019 ;
|Abstract||The gut microbiome influences the health and well-being of dogs. However, little is known
about the impact of breed on the fecal microbiome composition in dogs. Therefore, we aimed
to investigate the differences in the fecal microbiome in three breeds of dog fed and housed
under the same conditions, namely eight Maltese (8.0 ± 0.1 years), eight Miniature Schnauzer
(8.0 ± 0.0 years), and nine Poodle dogs (8.0 ± 0.0 years). Fresh fecal samples were collected
from the dogs and used to extract metagenomic DNA. The composition of the fecal
microbiome was evaluated by 16S rRNA gene amplicon sequencing on the MiSeq platform. A
total of 840,501 sequences were obtained from the 25 fecal samples and classified as Firmicutes
(32.3–97.3% of the total sequences), Bacteroidetes (0.1–62.6%), Actinobacteria (0.2–14.7%),
Fusobacteria (0.0–5.7%), and Proteobacteria (0.0–5.1%). The relative abundance of Firmicutes
was significantly lower in the Maltese dog breed than that in the other two breeds, while that
of Fusobacteria was significantly higher in the Maltese than in the Miniature Schnauzer breed.
At the genus level, the relative abundance of Streptococcus, Fusobacterium, Turicibacter,
Succinivibrio, and Anaerobiospirillum differed significantly among the three dog breeds. These
genera had no correlation with age, diet, sex, body weight, vaccination history, or parasite
protection history. Within a breed, some of these genera had a correlation with at least one
blood chemistry value. This study indicates that the composition of the fecal microbiome in
dogs is affected by breed.|
|Key_word||Dog, fecal microbiome, breed, dietary condition, bacterial abundance|
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