2019 ; Vol.29-10: 1543~1552
|Author||Wenli Xu, Jun Gao, Haoyue Zheng, Chaowen Yuan, Jinlong Hou, Liguo Zhang, Guoqing Wang|
|Place of duty||College of Life and Health Sciences, Northeastern University, Shenyang 110001, P.R. China|
|Title||Establishment and Application of Polymerase Spiral Reaction Amplification for Salmonella Detection in Food|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Salmonella is a common zoonotic and foodborne pathogen that causes high morbidity and
mortality in developing countries. In this study, we established and validated a polymerase
spiral reaction (PSR) assay which targeted the conserved invasion gene (invA) of Salmonella by
SYBR Green I indicator methods. Subsequently, assays for determination of the optimal
conditions for optimal specificity and sensitivity of PSR were performed. We performed
comprehensive evaluations using loop-mediated isothermal amplification (LAMP) and realtime
PCR. A total number of 532 samples of daily food were analyzed by PSR. Twenty-seven
bacterial strains were tested in the specificity assay, from which positive results were obtained
only for 14-Salmonella strains. However, none of the 13 non-Salmonella strains was amplified.
Similarly with LAMP and real-time PCR, the detection limit of the PSR assay was 50 CFU/ml.
The PSR method was also successfully applied to evaluate the contamination with Salmonella
in 532 samples of daily food, corroborating traditional culture method data. The novel PSR
method is simple, sensitive, and rapid and provides new insights into the prevention and
detection of foodborne diseases.|
|Key_word||Salmonella, invasion gene A, isothermal amplification, rapid detection, food samples|
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