2019 ; Vol.29-10: 1629~1635
|Author||Lamia Ayed, Tarek Zmantar, Sihem Bayar, Abdelkrim Cheref, Sami Achour, Hedi Ben Mansour, Ridha Mzoughi|
|Place of duty||Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, Route Avicenne, 5000 Monastir, Tunisia|
|Title||Potential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Azo dyes are recalcitrant pollutants, which are toxic, carcinogenic, mutagenic and teratogenic,
that constitute a significant burden to the environment. The decolorization and the
mineralization efficiency of Remazol Brillant Orange 3R (RBO 3R) was studied using a
probiotic consortium (Lactobacillus acidophilus and Lactobacillus plantarum). Biodegradation of
RBO 3R (750 ppm) was investigated under shaking condition in Mineral Salt Medium (MSM)
solution at pH 11.5 and temperature 25oC. The bio-decolorization process was further
confirmed by FTIR and UV-Vis analysis. Under optimal conditions, the bacterial consortium
was able to decolorize the dye completely (>99%) within 12 h. The color removal was 99.37%
at 750 ppm. Muliplex PCR technique was used to detect the Lactobacillus genes. Using
phytotoxicity, cytotoxicity, mutagenicity and biototoxicity endpoints, toxicological studies of
RBO 3R before and after biodegradation were examined. A toxicity assay signaled that
biodegradation led to detoxification of RBO 3R dye.|
|Key_word||Azo dye, mutagenicity, RBO 3R, biotoxicity, Lactobacillus, multiplex PCR|
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