Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-10: 1629~1635

AuthorLamia Ayed, Tarek Zmantar, Sihem Bayar, Abdelkrim Cheref, Sami Achour, Hedi Ben Mansour, Ridha Mzoughi
Place of dutyLaboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, Route Avicenne, 5000 Monastir, Tunisia
TitlePotential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-10
AbstractAzo dyes are recalcitrant pollutants, which are toxic, carcinogenic, mutagenic and teratogenic, that constitute a significant burden to the environment. The decolorization and the mineralization efficiency of Remazol Brillant Orange 3R (RBO 3R) was studied using a probiotic consortium (Lactobacillus acidophilus and Lactobacillus plantarum). Biodegradation of RBO 3R (750 ppm) was investigated under shaking condition in Mineral Salt Medium (MSM) solution at pH 11.5 and temperature 25oC. The bio-decolorization process was further confirmed by FTIR and UV-Vis analysis. Under optimal conditions, the bacterial consortium was able to decolorize the dye completely (>99%) within 12 h. The color removal was 99.37% at 750 ppm. Muliplex PCR technique was used to detect the Lactobacillus genes. Using phytotoxicity, cytotoxicity, mutagenicity and biototoxicity endpoints, toxicological studies of RBO 3R before and after biodegradation were examined. A toxicity assay signaled that biodegradation led to detoxification of RBO 3R dye.
Full-Text
Key_wordAzo dye, mutagenicity, RBO 3R, biotoxicity, Lactobacillus, multiplex PCR
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