Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-10: 1570~1579

AuthorYanjie Liu, Longfei Chen, Qiaohong Xie, Xi Yu, Anqing Duan, Yamin Lin, Biyun Xiang, Xiaoran Hao, Wanwan Chen, Xudong Zhu
Place of dutyBeijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, 100875, P.R. China
TitleA Gene Cluster for the Biosynthesis of Dibenzodioxocinons in the Endophyte Pestalotiopsis microspora, a Taxol Producer
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-10
AbstractThe fungal products dibenzodioxocinones promise a novel class of inhibitors against cholesterol ester transfer protein (CEPT). Knowledge as to their biosynthesis is scarce. In this report, we characterized four more dibenzodioxocinones, which along with a previously described member pestalotiollide B, delimit the dominant spectrum of secondary metabolites in P. microspora. Through mRNA-seq profiling in gα1Δ, a process that halts the production of the dibenzodioxocinones, a gene cluster harboring 21 genes including a polyketide synthase, designated as pks8, was defined. Disruption of genes in the cluster led to loss of the compounds, concluding the anticipated role in the biosynthesis of the chemicals. The biosynthetic route to dibenzodioxocinones was temporarily speculated. This study reveals the genetic basis underlying the biosynthesis of dibenzodioxocinone in fungi, and may facilitate the practice for yield improvement in the drug development arena.
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Supplemental Data
Key_wordDibenzodioxocinones, CEPT inhibitor, Pestalotiollide B, polyketide synthase, Pestalotiosis microspora NK17
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