2019 ; Vol.29-8: 1310~1315
|Author||Chaeri Park, Yun-Kyoung Song, Young Hyun Kim, Yena Jung, Young-Ho Park, Bong-Seok Song, Taekil Eom, Ju-Sung Kim, Sang-Hyun Kim, Ji-Su Kim, Sun-Uk Kim, Sang-Rae Lee, Ekyune Kim|
|Place of duty||College of Pharmacy, Catholic University of Daegu, Gyeongsan-si, Gyeongbuk, 38430, Republic of Korea|
|Title||Development of a New Type of Recombinant Hyaluronidase Using a Hexahistidine; Possibilities and Challenges in Commercialization|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier
to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available
hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform
encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and
assess its function. Therefore, in order to find the most efficient active hyaluronidase, we
produced several shortened hyaluronidases with partial removal of the N- or C-terminal
regions. Moreover, we created an enzyme that connected six histidines onto the end of the
hyaluronidase C-terminus. This simplified subsequent purification using Ni2+ affinity
chromatography, making it feasible to industrialize this highly active recombinant
hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme.
Therefore, this simple and effective isolation method could increase the availability of
recombinant hyaluronidase for research and clinical purposes.|
|Key_word||Recombinant, hyaluronidase, affinity chromatography, enzyme, hyaluronic acid|
Zhu Y, Kruglikov IL, Akgul Y, Scherer PE. 2019. Hyaluronan in adipogenesis, adipose tissue physiology and systemic metabolism. Matrix Biol. 78-79: 284-291.
Camaioni A, Salustri A, Yanagishita M, Hascall VC. 1996. Proteoglycans and proteins in the extracellular matrix of mouse cumulus cell-oocyte complexes. Arch. Biochem. Biophys. 325: 190-198.
Viola M, Karousou E, D'Angelo ML, Caon I, De Luca G, Passi A, et al. 2015. Regulated Hyaluronan Synthesis by Vascular Cells. Int. J. Cell Biol. 2015: 208303.
Aya KL, Stern R. 2014. Hyaluronan in wound healing:rediscovering a major player. Wound Repair Regen. 22: 579-593.
Nguyen N, Kumar A, Chacko S, Ouellette RJ, Ghosh A. 2017. Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities. Cell Commun. Signal. 15(1): 48.
Stern R, K ogan G , Jedrzejas M J, S oltes L. 2 007. T he m any ways to cleave hyaluronan. Biotechnol. Adv. 25: 537-557.
Nishihara T, Morimoto Y. 2017. Evaluation of transfer media containing different concentrations of hyaluronan for human in vitro fertilization. Reprod. Med. Biol. 16: 349-353.
Marei WF, Ghafari F, Fouladi-Nashta AA. 2012. Role of hyaluronic acid in maturation and further early embryo development of bovine oocytes. Theriogenology 78: 670-677.
Stern R, Jedrzejas MJ. 2006. Hyaluronidases: their genomics, structures, and mechanisms of action. Chem. Rev. 106: 818-839.
Kim E, Baba D, Kimura M, Yamashita M, Kashiwabara S, Baba T. 2005. Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass. Proc. Natl. Acad. Sci. U S A 102: 18028-18033.
Kimura M, Ishida K, Kashiwabara S, Baba T. 2009. Characterization of two cytoplasmic poly(A)-binding proteins, PABPC1 and PABPC2, in mouse spermatogenic cells. Biol. Reprod. 80: 545-554.
Yoon S, Chang KT, Cho H, Moon J, Kim JS, Min SH, et al. 2014. Characterization of pig sperm hyaluronidase and improvement of the digestibility of cumulus cell mass by recombinant pSPAM1 hyaluronidase in an in vitro fertilization assay. Anim. Reprod. Sci. 150: 107-114.
Myles DG, Primakoff P. 1984. Localized surface antigens of guinea pig sperm migrate to new regions prior to fertilization. J. Cell Biol. 99: 1634-1641.
Gmachl M, Kreil G. 1993. Bee venom hyaluronidase is homologous to a membrane protein of mammalian sperm. Proc. Natl. Acad. Sci. U S A 90: 3569-3573.
Park C, Kim YH, Lee SR, Park S, Jung Y, Lee Y, et al. 2018. Characterization of Recombinant Bovine Sperm Hyaluronidase and Identification of an Important Asn-XSer/Thr Motif for Its Activity. J. Microbiol. Biotechnol. 28: 1547-1553.
Geetha-Habib M, Park HR, Lennarz WJ. 1990. In vivo Nglycosylation and fate of Asn-X-Ser/Thr tripeptides. J. Biol. Chem. 265: 13655-13660.
Gabius HJ. 2018. T he s ugar c ode: W hy g lycans a re s o important. Biosystems 164: 102-111.