2019 ; Vol.29-7: 1104~1116
|Author||Hai The Pham, Hien Thi Tran, Linh Thuy Vu, Hien The Dang, Thuy Thu Thi Nguyen, Thu Ha Thi Dang, Mai Thanh Thi Nguyen, Huy Quang Nguyen, Hong Byung Kim|
|Place of duty||Research group for Physiology and Applications of Microorganisms (PHAM group), GREENLAB, Center for Life Science Research (CELIFE) and Department of Microbiology - Faculty of Biology, Vietnam National University – University of Science, Hanoi 144, Vietnam|
|Title||A Laboratory-Scale Study of the Applicability of a Halophilic Sediment Bioelectrochemical System for in situ Reclamation of Water and Sediment in Brackish Aquaculture Ponds: Establishment, Bacterial Community and Performance Evaluation|
J. Microbiol. Biotechnol.2019 ;
|Abstract||In this study, we investigated the potential of using sediment bioelectrochemical systems
(SBESs) for in situ treatment of the water and sediment in brackish aquaculture ponds polluted
with uneaten feed. An SBES integrated into a laboratory-scale tank simulating a brackish
aquaculture pond was established. This test tank and the control (not containing the SBES)
were fed with shrimp feed in a scheme that mimics a situation where 50% of feed is uneaten.
After the SBES was inoculated with microbial sources from actual shrimp pond sediments,
electricity generation was well observed from the first experimental week, indicating
successful enrichment of electrochemically active bacteria in the test tank sediment. The
electricity generation became steady after 3 weeks of operation, with an average current density of
2.3 mA/m2 anode surface and an average power density of 0.05 mW/m2 anode surface. The
SBES removed 20-30% more COD of the tank water, compared to the control. After 1 year, the
SBES also reduced the amount of sediment in the tank by 40% and thus could remove
approximately 40% more COD and approximately 52% more nitrogen from the sediment,
compared to the control. Insignificant amounts of nitrite and nitrate were detected, suggesting
complete removal of nitrogen by the system. PCR-DGGE-based analyses revealed the
dominant presence of Methylophilus rhizosphaerae, Desulfatitalea tepidiphila and Thiothrix
eikelboomii, which have not been found in bioelectrochemical systems before, in the bacterial
community in the sediment of the SBES-containing tank. The results of this research
demonstrate the potential application of SBESs in helping to reduce water pollution threats,
fish and shrimp disease risks, and thus farmers’ losses.|
|Key_word||Microbial fuel cell, sediment bioelectrochemical systems, brackish aquaculture, in situ bioremediation|
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