2019 ; Vol.29-7: 1137~1143
|Author||Jinjong Myoung, Kang Sang Min|
|Place of duty||Korea Zoonosis Research Institute and Genetic Engineering Research Institute, Chonbuk National University, Jeonju 54896, Republic of Korea|
|Title||Dose-Dependent Inhibition of Melanoma Differentiation-Associated Gene 5-Mediated Activation of Type I Interferon Responses by Methyltransferase of Hepatitis E Virus|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Hepatitis E virus (HEV) accounts for 20 million infections in humans worldwide. In most
cases, the infections are self-limiting while HEV genotype 1 infection cases may lead to lethal
infections in pregnant women (~ 20% fatality). The lack of small animal models has hampered
detailed analysis of virus-host interactions and HEV-induced pathology. Here, by employing a
recently developed culture-adapted HEV, we demonstrated that methyltransferase, a nonstructural
protein, strongly inhibits melanoma differentiation-associated gene 5 (MDA5)-
mediated activation of type I interferon responses. Compared to uninfected controls, HEVinfected
cells display significantly lower levels of IFN-β promoter activation when assessed by
luciferase assay and RT-PCR. HEV genome-wide screening showed that HEV-encoded
methyltransferase (MeT) strongly inhibits MDA5-mediated transcriptional activation of IFN-β
and NF-κB in a dose-responsive manner whether or not it is expressed in the presence/
absence of a tag fused to it. Taken together, current studies clearly demonstrated that HEV
MeT is a novel antagonist of MDA5-mediated induction of IFN-β signaling.|
|Key_word||Hepatitis E virus, interferon beta, methyltransferase|
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