Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-8: 1204~1211

AuthorJuhui Jin, Thi Thanh Hanh Nguyen, Changmu Kim, Doman Kim
Place of dutyGraduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea
TitleAntimelanogenesis Effects of Fungal Exopolysaccharides Prepared from Submerged Culture of Fomitopsis castanea Mycelia
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-8
AbstractFungal exopolysaccharides are important natural products having diverse biological functions. In this study, exopolysaccharides from Fomitopsis castanea mycelia (FEPS) were prepared, and the highest mushroom tyrosinase inhibitory activity was found. FEPS were prepared from cultivation broth by ethanol precipitation method. The extraction yield and protein concentration of FEPS were 213.1 mg/l and 0.03%, respectively. FEPS inhibited mushroom tyrosinase with the half maximal inhibitory concentration (IC50) of 16.5 mg/ml and dose-dependently inhibited cellular tyrosinase activity (63.9% at 50 μg/ml, and 83.3% at 100 μg/ml) in the cell-free extract of SK-MEL-5 human melanoma cell and α-melanocytestimulating hormone (α-MSH)-stimulated melanin formation in intact SK-MEL-5 human melanoma cell. The IC50 of FEPS against NO production from RAW264.7 macrophage cells was 42.8 ± 0.64 μg/ml. By in vivo study using a zebrafish model, exposure of FEPS at 400 μg/ml to dechorionated zebrafish embryos for 18 h decreased the pigment density, compared to that without FEPS-treated control
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Key_wordFomitopsis castanea, exopolysaccharides, SK-MEL-5 human melanoma cells, zebrafish, tyrosinase
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