Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-9: 1488~1493

AuthorHuofeng Jiang, Yamin Gao, Sheng Zeng, Shuai Wang, Zhizhong Cao, Yaoju Tan, Huancai Yin, Jianxiong Liu, Tianyu Zhang
Place of dutySchool of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, P.R. China.,State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, P.R. China.
TitleOne-Step Engineering of a Stable, Selectable Marker-Free Autoluminescent Acinetobacter baumannii for Rapid Continuous Assessment of Drug Activity
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-9
AbstractThe rising cases of multidrug-resistant Acinetobacter baumannii (Ab) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/dif system, we constructed a stable, selectable marker-free autoluminescent Ab capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti-Ab drug candidates in vitro.
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Key_wordAutoluminescent, marker-free, Acinetobacter baumannii, Tn7, Xer/dif recombination system
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