2019 ; Vol.29-8: 1193~1203
|Author||Su-Hyeon Cho, Soo-Jin Heo, Hye-Won Yang, Eun-Yi Ko, Myeong Seon Jung, Seon-Heui Cha, Ginnae Ahn, You-Jin Jeon, Kil-Nam Kim|
|Place of duty||Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of Korea,Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea|
|Title||Protective Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against Hydrogen Peroxide-Induced Oxidative Stress In Vitro and In Vivo|
J. Microbiol. Biotechnol.2019 ;
|Abstract||We investigated the protective effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) from
Polysiphonia morrowii Harvey against hydrogen peroxide (H2O2)-induced apoptosis in Vero
cells. BDB exhibited scavenging activity for DPPH, hydroxyl, and alkyl radicals. BDB also
inhibited H2O2-induced lipid peroxidation, cell death, and apoptosis in Vero cells by inhibiting
the production of ROS. To evaluate the molecular mechanisms of apoptosis inhibition, the
expression of Bax/Bcl-xL and NF-κB was assessed by western blot assay. BDB significantly
suppressed the cleavage of caspase-9 and PARP and reduced Bax levels in H2O2-induced Vero
cells. Besides, BDB suppressed the phosphorylation of NF-κB and the translocation of p65 in
H2O2-induced cells. Furthermore, we evaluated the effect of BDB on ROS production, cell
death, and lipid peroxidation in an H2O2-stimulated zebrafish embryo model. Taken together,
these results indicated that ROS generation and cell death were significantly inhibited by BDB
in zebrafish embryos, thereby proving that BDB exerts excellent antioxidant activity in vitro
and in vivo.|
|Key_word||Polysiphonia morrow Harvey, 3-bromo-4,5-dihydroxybenzaldehyde, reactive oxygen species, oxidative stress, antioxidant, zebrafish embryos|
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