2019 ; Vol.29-9: 1401~1411
|Author||Chaebok Lee, Bindu Subhadra, Hei-Gwon Choi, Hyun-Woo Suh, Han S. Uhm, Hwa-Jung Kim|
|Place of duty||Department of Medical Science, Chungnam National University, Daejeon 35015, Republic of Korea,Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea|
|Title||Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Mycobacterial cell walls comprise thick and diverse lipids and glycolipids that act as a
permeability barrier to antibiotics or other chemical agents. The use of OH radicals from a
non-thermal plasma jet (NTPJ) for the inactivation of mycobacteria in aqueous solution was
adopted as a novel approach. Addition of water vapor in a nitrogen plasma jet generated OH
radicals, which converted to hydrogen peroxide (H2O2) that inactivated non-pathogenic
Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis H37Rv. A stable plasma
plume was obtained from a nitrogen plasma jet with 1.91 W of power, killing Escherichia coli
and mycobacteria effectively, whereas addition of catalase decreased the effects of the former.
Mycobacteria were more resistant than E. coli to NTPJ treatment. Plasma treatment enhanced
intracellular ROS production and upregulation of genes related to ROS stress responses (thiolrelated
oxidoreductases, such as SseA and DoxX, and ferric uptake regulator furA).
Morphological changes of M. smegmatis and M. tuberculosis H37Rv were observed after 5 min
treatment with N2+H2O plasma, but not of pre-incubated sample with catalase. This finding
indicates that the bactericidal efficacy of NTPJ is related to the toxicity of OH and H2O2
radicals in cells. Therefore, our study suggests that NTPJ treatment may effectively control
pulmonary infections caused by M. tuberculosis and nontuberculous mycobacteria (NTM) such
as M. avium or M. abscessus in water.|
|Key_word||Non-thermal plasma, hydroxyl radical, ROS stress, Mycobacteria|
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