2019 ; Vol.29-7: 1071~1077
|Author||Shimin Guan, Shaofeng Rong, Mengze Wang, Baoguo Cai, Qianqian Li, Shuo Zhang|
|Place of duty||Department of Biological Engineering, Shanghai Institute of Technology, Shanghai 201418, P.R. China|
|Title||Enhanced Biotransformation Productivity of Gamma-Decalactone from Ricinoleic Acid Based on the Expanded Vermiculite Delivery System|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Natural gamma-decalactone (GDL) produced by biotransformation is an essential food
additive with a peach-like aroma. However, the difficulty of effectively controlling the
concentration of the substrate ricinoleic acid (RA) in water limits the biotransformation
productivity, which is a bottleneck for industrialization. In this study, expanded vermiculite
(E-V) was utilized as a carrier of RA to increase its distribution in the medium. E-V and three
commonly used organic compounds were compared with respect to their effects on the
biotransformation process, and the mechanism was revealed. Scanning electron microscopy,
Fourier transform infrared spectroscopy and thermogravimetric analysis indicated that RA
was physically adsorbed onto the surface of and inside E-V instead of undergoing a chemical
reaction, which increased the opportunity for interactions between microorganisms and the
substrate. The highest concentration of GDL obtained in the medium with E-V was 6.2 g/l,
which was 50% higher than that in the reference sample. In addition, the presence of E-V had
no negative effect on the viability of the microorganisms. This study provides a new method
for producing natural GDL through biotransformation on an industrial scale.|
|Key_word||Biotransformation, gamma- decalactone, ricinoleic acid, expanded vermiculite|
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