2019 ; Vol.29-9: 1453~1459
|Author||Minjae Kim, Yongsoo Kang, EonSeon Jin|
|Place of duty||Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea|
|Title||Gene Expression Analysis of Zeaxanthin Epoxidase from the Marine Microalga Dunaliella tertiolecta in Response to Light/Dark Cycle and Salinity|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Zeaxanthin is an important pigment in the photo-protection mechanism of microalgae.
However, zeaxanthin epoxidase, an enzyme involved in the accumulation and conversion of
zeaxanthin, has not been extensively studied in microalgae. In this work, we report the
expression pattern of zeaxanthin epoxidase in Dunaliella tertiolecta (DtZEP) at different light
and diverse salinity conditions. To confirm the responsiveness to light conditions, the ZEP
expression pattern was investigated in photoperiodic (16 h of light and 8 h of dark) and
continuous (24 h of light and 0 h of dark) light conditions. mRNA expression levels in
photoperiodic conditions fluctuated along with the light/dark cycle, whereas those in
continuous light remained unchanged. In varying salinity conditions, the highest mRNA and
protein levels were detected in cells cultured in 1.5 M NaCl, and ZEP expression levels in cells
shifted from 0.6 M NaCl to 1.5 M NaCl increased gradually. These results show that mRNA
expression of DtZEP responds rapidly to the light/dark cycle or increased salinity, whereas
changes in protein synthesis do not occur within a short period. Taken together, we show that
DtZEP gene expression responds rapidly to light irradiation and hyperosmotic stress. In
addition, ZEP expression patterns in light or salinity conditions are similar to those of higher
plants, even though the habitat of D. tertiolecta is different.|
|Key_word||Zeaxanthin epoxidase, Dunaliella tertiolecta, carotenoid biosynthetic pathway, photoperiodic condition, continuous light condition, salinity condition|
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